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Identifying experimental surrogates for Bacillus anthracis spores: a review
Investigative Geneticsvolume 1, Article number: 4 (2010)
Bacillus anthracis, the causative agent of anthrax, is a proven biological weapon. In order to study this threat, a number of experimental surrogates have been used over the past 70 years. However, not all surrogates are appropriate for B. anthracis, especially when investigating transport, fate and survival. Although B. atrophaeus has been widely used as a B. anthracis surrogate, the two species do not always behave identically in transport and survival models. Therefore, we devised a scheme to identify a more appropriate surrogate for B. anthracis. Our selection criteria included risk of use (pathogenicity), phylogenetic relationship, morphology and comparative survivability when challenged with biocides. Although our knowledge of certain parameters remains incomplete, especially with regards to comparisons of spore longevity under natural conditions, we found that B. thuringiensis provided the best overall fit as a non-pathogenic surrogate for B. anthracis. Thus, we suggest focusing on this surrogate in future experiments of spore fate and transport modelling.
Bacillus anthracis, the causative agent of anthrax, has received much attention in the past decade due to its use in 2001 as a biological weapon distributed through the USA mail system. However, B. anthracis spores have been used as a weapon for close to 100 years and, historically, this pathogen was an important disease model . This bacterium also provides a nearly perfect model of prokaryotic clonal evolution, with rare genomic recombination and extremely low levels of homoplasy . The body of research acquired for B. anthracis provides key insights into its biology, epidemiology and the risks associated with its release into a civilian environment . However, an important gap still remains in our empirical understanding of B. anthracis spore survival and mobility. As a result, it is necessary to examine and develop more accurate fate and transport models of anthrax spores in order to better understand public health risks and develop methods for emergency response to a mass release.
Mathematical fate and transport models provide a means of predicting the distribution of pathogenic particles after their release into air or water. Clearly, such information is an important asset in risk assessment following a terrorist attack or a biological accident. Scenarios for intentional release into a civilian area include infecting the water supply or releasing aerosolized spores [4, 5]. In a 1970 report, the World Health Organization predicted that 50 kg of spores released upwind of 500,000 civilians would result in 95,000 fatalities; likewise, a single subway attack could lead to over 10,000 deaths if carried out during rush hour . Model scenarios and the 2001 events demonstrate that non-targeted individuals are also vulnerable. However, models may lack predictive power if their critical parameters are not based on real world values. Therefore, it is necessary to collect experimental data that will lead to greater model accuracy of spore behaviour. For example, our laboratory group is performing experiments to measure attenuation values for spore survivability in natural and artificial environments (such as water, soil and fomites). These and other experiments will help to validate the predictions of current mathematical models, thereby increasing model accuracy and improving our response to natural, accidental or intentional releases of anthrax.
Fully virulent B. anthracis must be handled under biosafety level (BSL)-3 conditions and requires secure containment. Therefore, we cannot experimentally release this organism into the environment nor use it in experiments outside of a BSL3 facility. In order to conduct experiments that inform release models, we must use a non-pathogenic bacterium that can accurately represent B. anthracis. Surrogates of this type have been used for many years in military release experiments, water supply studies and food protection assessment. However, little attention has been focused on the criteria used to select surrogates. Our synthesis makes use of existing empirical evidence to present an informed decision for the best choice of a B. anthracis surrogate.
History of surrogate use for B. anthracis
Before selecting an appropriate surrogate for B. anthracis, it is useful to review the history of surrogate use for this organism. This information, though anecdotal in some cases, provides valuable information useful for surrogate selection such as (1) comparative survival and behavioural data, (2) an initial list of potential surrogate candidates and (3) baseline data to compare against current experiments. Over the years a number of surrogates have been used, including an attenuated B. anthracis strain (Sterne) and several phylogenetic relatives: B. atrophaeus (formerly B. globigii and B. subtilis niger[7, 8]), B. cereus, B. megaterium, B. mycoides, B. subtilis, B. thuringiensis and Geobacillus (Figure 1). Table 1 indicates the number of times each has been utilized in published studies. B. atrophaeus has been employed most frequently; B. cereus, B. subtilis and B. thuringiensis have been used moderately; and the others have been used just a few times (B. megaterium, B. mycoides and Geobacillus).
Both the USA and Japanese governments used pathogenic simulants in biological warfare test studies. For example, Yoshi Iishi of Japan confessed after World War II to using B. anthracis surrogates in his biological warfare programme, which was initiated in 1935 . The USA began using B. atrophaeus as their major non-pathogenic surrogate for B. anthracis in July of 1943 at Camp Detrick . This surrogate has been used for many experiments in order to ascertain potential outcomes of using anthrax as a biological weapon [10–12]. In 1949 the USA Army experimentally sprayed B. atrophaeus and Serratia marcescens over the coastal population centers of Hampton, Virginia and San Francisco, California . B. atrophaeus was also disseminated in Greyhound bus and New York subway terminals via covert spray generators hidden in briefcases during the mid-1960 s . More recent work at national laboratories has emphasized the detection and identification of spores in the environment.
The earliest in-depth comparison of related Bacillus species was done by Schneiter and Kolb [13, 14], who tested heat processing methods to destroy 'industrial' spores of B. anthracis, B. subtilis and B. cereus found on shaving brush bristles. Brazis et al. made a direct comparison of the effect of free available chlorine on B. anthracis and B. atrophaeus spores and found that B. atrophaeus was more resistant to chlorine. In these early works, no mention is made of the potential for these species to be used as B. anthracis surrogates. However, their results provide valuable comparative data (for example, B. atrophaeus is more resistant to chlorine and therefore is a conservative surrogate for estimating B. anthracis survival in tap water).
More recent experiments have examined the effects of various environmental challenges and disinfectants on B. anthracis surrogates, including studies of food protection or decontamination in the wake of a release event. Faille et al. used B. thuringiensis as a non-pathogenic representative for B. cereus and indicated that B. thuringiensis has been used in this capacity for many years. Others have used B. atrophaeus, B. thuringiensis, B. cereus and B. subtilis to examine decontamination strategies using various bactericidal compounds such as chlorine, hydrogen peroxide, dyes, neutral oxone chloride, formaldehyde, gluteraldehyde and antibiotics [15, 17–43]. Additional decontamination methods used against these surrogates include ultraviolet irradiation [39, 44–50], plasma , electron beam radiation [52, 53] and heat [39, 54–63].
B. anthracis stand-ins have also played an important role in evaluating the broad arsenal of techniques used to detect and identify bio-threat agents in the environment. At least 17 methods have been employed to detect spores of B. anthracis and its relatives, including: electron microscopy , atomic force microscopy [65–68], photothermal spectroscopy , microcalorimetric spectroscopy , biochip sensors [71, 72], Raman spectroscopy , polymerase chain reaction methods [74–80], optical chromatography , differential mobility spectroscopy , laser induced breakdown spectroscopy [83–86], flow cytometry sorting , mass spectroscopy [88–96], proteomics [97, 98], luminescence analysis , long-wave biosensors , lytropic liquid sensors  and fluorescent labelling [102–105]. Although most of these studies used B. anthracis directly, some included close relatives for comparisons of detectability across species.
Lastly, surrogates have played an important role in several types of aerosol studies. They have been used to evaluate electrical forces [106, 107], examine the effect of filter material on bioaerosol collection  and to determine if bees could be deployed to detect anthrax spores in the air . Other studies have used stand-ins such as B. thuringiensis to test spore movement in aerial spray [4, 110, 111], transport and deposition efficiency of spores in ventilation ducts , engineered aerosol production  and re-aerosolization of spores . B. atrophaeus has been used to reproduce an anthrax letter event, demonstrating how an individual swine located 1.5 m from an opened letter inhaled >21,000 spores . This is a lethal dose for humans exposed to B. anthracis and validates the significant biothreat of passive spore dispersion.
From the diverse experimental uses of anthrax surrogates during the last 70 years, it is obvious that non-pathogenic representatives are indispensable for conducting safe inquiries into the behaviour and mobility of pathogen spores. However, not all species are equally appropriate stand-ins for B. anthracis. In the remainder of this review we outline our selection criteria, present pertinent literature for surrogate selection in B. anthracis and identify gaps in our knowledge of a surrogate's ability to mimic the behaviour of this pathogen. Whenever possible, we present quantified values to provide robust justification of any surrogate to be used in future fate and transport experiments.
We used several criteria for selection, including (1) the risk of use (pathogenicity), (2) genetic similarity to B. anthracis, (3) morphology and (4) response to various chemical and environmental challenges. Our initial list began with microbes in the family Bacillaceae that have been used as surrogates in the past. Practical attributes of potential surrogates are summarized in Table 2. It is important to select appropriate representatives with regard to the specific experiments one wishes to conduct. As an example, if we were interested in studying the disinfectant capacity of a substance we would use a surrogate that has greater survivability than our target organism. The results would then provide conservative estimates of appropriate disinfectant levels. In our case, we are interested in physical experiments of mobility in water and air media. Hence, we determined that the physical properties of the spores are of greatest interest, including size, shape, density, surface morphology, surface structure and surface hydrophobicity. Behavioural responses to stress and natural conditions are also relevant to spore survival.
The risks associated with surrogate use are of critical concern. Table 3 lists the biosafety designations for the potential surrogates. Surrogates are typically used to replace a pathogen that, if used, would present a potential threat to public health. B. anthracis is classified as a BSL-3 organism and work must be conducted under highly contained conditions not suitable for fate and transport experiments. Ideally, an attenuated strain of B. anthracis would be a good surrogate because it should behave similarly to the pathogenic strains and pose little risk. However, our knowledge of plasmid exchange rates and the environmental effects of these strains remains very limited - they may still pose a risk despite being classified as BSL-2 organisms. In addition, detection of B. anthracis in the environment, even of an attenuated strain, could cause a public relations issue. Worse, released surrogates might mask a real attack or create high background positives and unnecessary emergency responses. Therefore, we feel that non-pathogenic B. anthracis strains are not good surrogates for fate and transport experiments.
Another surrogate of interest is B. cereus. This species is an opportunistic food-borne pathogen that can infect humans [116, 117] and the CDC recommends the handling of the organism at BSL-2 standards. Although it is naturally found in the environment, additional releases of this potential pathogen are deemed unsafe. As such, this organism cannot be used as a replacement for B. anthracis in spore release studies. The same is true for B. megaterium and Geobacillus stearothermophilus, which are treated as BSL-2 organisms.
The other potential surrogates, including B. atrophaeus, B. mycoides, B. subtilis and B. thuringiensis, are not typically regarded as potential human pathogens or select agents. They are BSL-1 organisms and are safe candidates. B. thuringiensis is used as an insecticide throughout the world, and has been shown to pose no health risk to humans in some studies [118, 119]. Infections do occasionally occur, however. These include a case from using commercial B. thuringiensis var. kurstaki, a wound infection identified as B. thuringiensis strain 97-27 [74, 121], and an isolate recovered from a gastrointestinal illness . That said, the overall the use of most B. thuringiensis strains appears to be safe and this species provides a good potential surrogate for B. anthracis[118, 119]. B. atrophaeus is commonly found in soil throughout the world, is considered non-pathogenic and has been used extensively as a surrogate for B. anthracis[40, 123]. B megaterium and B. subtilis are also found in the soil and are non-pathogenic to humans. Based on safety concerns, most candidates except B. cereus could serve as a surrogate for B. anthracis.
Genetics of the potential surrogates
Genetic relationships are important when selecting a surrogate because, theoretically, a phylogenetic relative should be morphologically and behaviorally more similar and have comparable physical characteristics to the target organism. There have been many genetic studies that elucidate the phylogenetic relationships of organisms related to B. anthracis[74, 98, 124–143]. The results of these studies indicate that B. anthracis is most closely related to B. cereus, B. thuringiensis and B. mycoides, which are grouped together as the B. cereus group (Figure 1). In contrast, B. subtilis, B. atrophaeus, B. megaterium, and Geobacillus are more distant relatives of B. anthracis. As their chromosomal genomes are very similar, some authors have suggested that B. cereus, B. thuringiensis and B. anthracis are actually a single species separated only by different plasmid composition . However, highly informative genetic markers such as single nucleotide polymorphisms can resolve B. anthracis from these near neighbor species [144, 145]. The identification of closely related surrogates does not present a problem when these powerful genetic tools are used. The importance of genetic similarity on spore composition is demonstrated by the BclA gene, which is unique to the B. cereus group. This protein is found in the exosporium and helps determine the adhesive properties of the spore [146, 147]. As B. atrophaeus and B. megaterium are lacking this gene, we would expect important changes in behavior compared to B. anthracis.
Morphology of the potential surrogates
Morphological characters are important to consider when choosing a surrogate because physical behaviours are the foundation of transport models. As stated earlier, genetic relatedness is a good indicator of morphological similarity, so we expect organisms within the B. cereus group to be morphologically similar to B. anthracis. Microscopy examination reveals few morphological features that can be used to definitively distinguish the various species from one another [64, 65, 68]. However, spores present measurable differences among surrogates, including the structure of the exosporium, the presence/absence of filamentous appendages and size variation.
The spores of the B. cereus group all possess a specific type of exosporium surrounding the outer spore coat. It is a balloon-like sac that envelopes the spore, is made of crystal lattices and, typically, has a short nap of hair-like projections extending off the surface [64–68, 146, 148–154]. The exosporium can be highly variable, both among B. anthracis relatives [155–157] and within B. anthracis, as shown by differences between the Vollum and Sterne strains . Some species also have long appendages that extend off the exosporium, known as filaments. B. cereus, B. megaterium and B. thuringiensis all possess filaments, whereas B. anthracis has none [64, 149–152, 158–161]. More distant relatives such as B. atrophaeus and B. subtilis have neither a nap nor filaments [67, 68, 152, 162]. Likewise, B. atrophaeus and B. megaterium have an atypical exosporium-like layer that is distinct but does not extend off the surface of the outer coat [64, 67, 148, 152, 162–165]. B. thuringiensis has a similar nap to B. anthraci s but the presence or absence of filaments in B. thuringiensis is variable [152, 166–168]. It is important to note that the exosporium is strongly hydrophobic  and that this chemical property may influence flow dynamics in aqueous solutions. Therefore, species with less hydrophobic spores (B. subtilis) are probably not appropriate simulants compared to the B. cereus group. As differences in exterior morphology will influence the mobility of pathogen spores in air and water, the investigation of these dynamics is a much-needed focus of future research.
Size, shape and density of the spore are also considered important factors that can influence surrogate behavior in release experiments. The spores of the B. cereus group have similar ratios of length to width and similar diameters, whereas the spores of B. atrophaeus are smaller and those of B. megaterium are larger [65, 68, 170, 171]. Although the difference in size is not great, it does exist and may require different coefficients for various model parameters (such as, Reynolds number, diffusion coefficient and sedimentation velocity) [172, 173]. Spore volume is strongly correlated to density (R = 0.95) when spores are wet and in a moistened state the smaller spores of B. atrophaeus and B. subtilis are much more dense than B. anthracis. Such differences are likely to affect the behaviour of these particles in air or water. Wet B. thuringiensis spores have densities and volumes within the range of B. anthracis, making this simulant a better match for the measurement of liquid dispersion. Interestingly, dry spore density is similar among the surrogates listed in Table 1, despite volume differences . Thus, the right choice of surrogate appears to depend on the dispersion medium under consideration.
Comparative survivability among surrogates
Previous experiments comparing the survivability of various spore-formers provide valuable information to the surrogate selection process. Comparative experiments of spore survival under natural conditions or exposure to heat, ultraviolet and chemical disinfectants can illuminate which species may behave similarly to B. anthracis in experiments. In this section we review the literature for comparative spore survival.
Quantitative data relating inactivation kinetics of the natural survival of spores would be of great value when comparing potential surrogates. Unfortunately, most of the available data are qualitative. Past studies with B. anthracis have revealed that spores may survive for years under natural conditions [175–190]. The data are mostly qualitative, not directly comparable, and primarily exist only for B. anthracis. Experimental evidence that quantifies survival rates in both the short and long term are missing. Several studies examined the attenuation rate of B. thuringiensis spores on leaves, soil and snow [191–197]; B. cereus was included in a survival study measuring the effects of soil pH, moisture, nutrients and presence of other microbes . In addition to two aerosol field studies [110, 199], we found no other studies that investigated natural attenuation rates of the potential surrogates for B. anthracis or that compared several species at once. Another drawback to using these data is that spore behaviour is variable due to factors such as purification method, sporulation conditions and strain type, and in many of these studies different purification protocols and strains are used, which makes direct comparisons of the values mostly pointless. Nevertheless these values do have some comparative information that can be used for surrogate selection. For example, natural attenuation values have been quantified for B. cereus and B. thuringiensis demonstrating that, after 135 days, the number of viable B. thuringiensis spores falls to about a quarter of the original inoculum . The same may be true for B. anthracis but data are lacking. Although some spores remain active for a long time, the rate at which they lose viability is unknown, which suggests that additional experimental evidence is required to confirm the decay rates for B. anthracis spores and the potential surrogates.
Many experiments have been conducted that examine the effects of heat on spores [39, 54, 57, 63, 200–208]. However, very few studies have focused on quantifying differences in the survival of spores with regards to surrogate selection. More recent studies have compared the affect of heat on spores with the intention to understand differences among species. The main focus of most of these experiments is related to industrial sanitation, particularly disinfection in the food industry [58–60, 62, 209–211]. Montville and coworkers  have published the only study that specifically compares attenuation values among several surrogates. Whitney et al. review some of the studies on the thermal survival of B. anthracis, whereas Mitscherlich and March  provide a very comprehensive review on the overall survival of B. anthracis and many of the potential surrogate candidates. However, it is apparent that the variability of D values (decimal reduction times) within species is large enough that we cannot make any robust decisions based upon this comparative information . Rather, from these data we realize that each strain may behave differently with regards to survivability. As a result, each potential surrogate species should be compared directly with B. anthracis in future experimental studies.
Experiments to compare the effect of disinfectants can also be useful for examining parallels in spore resilience. Whitney et al. reviewed many of the studies that have performed disinfectant trials on B. anthracis. Brazis et al. compared the effects of chlorine on B. atrophaeus and B. anthracis spores and found B. atrophaeus survival to be a conservative indicator for B. anthracis survival. B. cereus spores reasonably simulate B. anthracis spore inactivation by peroxyacetic acid-based biocides, but are less reliable for hydrogen peroxide, sodium hypochlorite, and acidified sodium chlorite . Rice et al. examined the affect of chlorine on several B. anthracis strains and potential surrogates and found that B. thuringiensis behaviour was most similar to a virulent B. anthracis strain. However, they also found a difference between the attenuated and virulent B. anthracis strains, indicating that even very close organisms may behave differently when conditions vary. More recently, Sagripanti et al. investigated the effects of various chlorides and other decontaminants on virulent B. anthracis and several potential surrogates on glass, metal, and polymeric surfaces.
Over the years many studies have focused on different bactericidal techniques for B. anthracis and their comparative effect on survival, including ultraviolet [44, 48–50, 214] and various chemicals [15, 34, 39, 215]. Two of the ultraviolet studies were geared toward surrogate selection. Nicholson and Galeano  validated B. subtilis as a good ultraviolet surrogate for B. anthracis using the attenuated Sterne strain. However, another study found B. subtilis spores were highly resilient to ultraviolet ionizing radiation when immersed in water and concluded this species would be a poor surrogate for B. anthracis. Menetrez and coworkers  found that B. anthracis Sterne was more resistant to ultraviolet than other surrogates, including B. thuringiensis, B. cereus and B. megaterium. Therefore, the data remain equivocal for choosing a stand-in with similar ultraviolet survival characteristics.
The results from the literature search on survivability are useful, but must be used with caution when comparing surrogates. Several authors have noted the high variability observed between spore batches and experiments [26, 44]. This variability makes the translation of results from different researchers difficult. Stringent testing of differences between strains can only take place when careful experimental designs are employed, including sporulation under identical conditions and strictly conserved methods for purification and survival estimates. The overall conclusions drawn from the results of previous survivability experiments suggest that any of our potential surrogates may behave similarly to B. anthracis. As a result, individual laboratory testing is also required in order to empirically validate a surrogate choice based on theoretical considerations.
Choice of surrogate
Our goal was to examine the various possible surrogates for B. anthracis, review the criteria for selecting an appropriate surrogate, compare the potential surrogates by these criteria and, ultimately, choose the most appropriate surrogate for our purposes. After examination of the first criteria, safety of use, we are left with B. atrophaeus, B. thuringiensis, B. megaterium and B. subtilis as potential surrogates. However, after further examination of genetic relatedness and the consequential morphological differences, B. thuringiensis emerges as the most appropriate candidate for a B. anthracis surrogate. This may be a surprising choice for some researchers, based on the traditional preference for B. atrophaeus. However, further examination of published comparisons also supports B. thuringiensis as a good surrogate for B. anthracis.
We recommend B. thuringiensis as the most appropriate surrogate based upon existing empirical data. As a result of the phenotypic similarity within the B. cereus group it will be important to utilize a B. thuringiensis strain that has a publically available genome sequence, such as B. thuringiensis serovar israelensis (ATCC 35646; GenBank No. AAJM01000000). This will allow for strain-specific markers to be identified [217, 218] which can be used as the basis for assays that can readily detect this strain and distinguish it from con-specifics as well as near neighbour species. We stress that additional experimental evidence is needed to confirm that B. thuringiensis and B. anthracis have similar behaviours. Data on spore survival and mobility are extremely lacking and we have identified several important knowledge gaps (Table 4). We have found only a few studies comparing spores from Bacillus species with the goal of surrogate validation and comparison [26, 40, 44, 48, 60]. We are aware of no studies that provide comparative survival of the surrogate candidates in soil or on different types of fomites, both under natural conditions and with heat, pH variance or UV radiation. In addition, there are no quantitative studies on the long-term survival of the spores in any medium. We also find very few studies that use virulent B. anthracis strains. The current literature suggests that there can be differences between the attenuated strains and the virulent strains. Therefore, in order to truly quantify and thereby confirm that our selected surrogate is the correct choice, we recommend conducting additional comparative experiments.
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This study was supported by the Center for Advancing Microbial Risk Assessment, which is funded by the US Environmental Protection Agency Science to Achieve Results programme and the US Department of Homeland Security University Programs (grant R3236201).
The authors declare that they have no competing interests.
DG and DW conceived the study. DG, JB, PK and DW drafted the manuscript. All authors read and approved the final manuscript.
Authors’ original submitted files for images
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