Figure 2

Distribution of the length of human mtDNA reads from sample ID 8727C. In total, 277,470 reads were trimmed to remove primers and the resulting 218,102 mapped (to the Reconstructed Sapiens Reference Sequence) reads were filtered to remove duplicates as a result of clonal reads. The resulting 7,756 unique mapped reads (2.8% of the total reads) gave 99.5% mitochondrial genome sequence (16,487 base pairs) coverage. The mean fold coverage was 20x (SD ± 9.7), mean unique read length was 43.2 bp (SD ± 9.3) and all fragments captured were <80 bp.