Development of a fast PCR protocol enabling rapid generation of AmpFℓSTR® Identifiler® profiles for genotyping of human DNA

Table 2 Stutter percentages calculated for Identifiler STR loci amplified using the fast PCR protocol.

		Fast-PCR protocol						Applied Biosystems Standard protocol^a
	Marker	N^b	Average (%)	SD (%)	Average + 2 SD (%)	Average + 3 SD (%)	Maximum (%)	Maximum (%)
n-4	D8S1179	165	7.4	1.4	10.1	11.5	12.5	8.2
	D21S11	188	8.5	1.3	10.9	12.2	13.1	9.4
	D7S820	168	5.5	1.8	8.8	10.6	11.8	8.2
	CSF1PO	151	6.0	1.4	8.7	10.1	12.6	9.2
	D3S1358	156	8.6	1.5	11.6	13.1	13.3	10.7
	THO1	174	2.5	0.9	4.2	5.1	4.9	5.1
	D13S317	164	4.8	2.1	9.2	11.3	12.4	8.0
	D16S539	160	6.6	1.7	10.2	11.9	11.1	10.4
	D2S1338	219	9.8	2.2	14.3	16.6	18.1	11.1
	D19S433	159	8.9	1.4	11.6	13.0	12.7	13.3
	vWA	176	8.4	2.4	13.6	16.0	21.1	12.6
	TPOX	179	3.6	1.4	6.0	7.3	9.8	4.8
	D18S51	198	9.2	2.7	14.5	17.2	18.1	17.0
	D5S818	145	7.3	1.2	9.7	10.9	11.1	6.8
	FGA	176	8.9	2.6	13.6	16.1	17.5	14.7
n+4 ^c	D8S1179	73	0.8	0.3	1.3	1.6	1.6	-
	D21S11	61	1.3	0.7	2.5	3.2	4.2	-
	D7S820	18	1.0	0.6	2.0	2.6	2.9	-
	CSF1PO	36	1.0	0.6	2.0	2.6	3.6	-
	D3S1358	22	1.3	0.5	2.1	2.6	2.3	-
	THO1	6	1.2	0.9	2.7	3.6	3.0	-
	D13S317	22	1.0	0.5	1.9	2.4	2.4	-
	D16S539	18	1.2	0.7	2.3	3.0	3.2	-
	D2S1338	8	1.1	0.5	2.0	2.5	1.9	-
	D19S433	1	1.2	-	1.2	1.2	1.2	-
	vWA	21	1.7	0.6	2.9	3.5	2.9	-
	TPOX	17	1.9	0.7	3.5	4.2	3.1	-
	D18S51	13	1.7	0.7	2.8	3.4	3.2	-
	D5S818	9	2.0	1.6	4.6	6.2	5.0	-
	FGA	0	-	-	-	-	-	-
n-8 ^c	D8S1179	28	0.8	0.2	1.2	1.4	1.3	-
	D21S11	37	1.0	0.3	1.6	1.9	1.9	-
	D7S820	3	0.8	0.6	1.7	2.3	1.5	-
	CSF1PO	11	0.6	0.2	1.0	1.2	1.0	-
	D3S1358	16	1.2	0.3	1.7	2.0	1.9	-
	THO1	0	0.0	-	-	-	-	-
	D13S317	5	0.6	0.1	0.8	0.9	0.7	-
	D16S539	9	0.9	0.3	1.4	1.6	1.4	-
	D2S1338	55	1.4	0.4	2.3	2.7	2.3	-
	D19S433	16	1.4	0.7	2.5	3.2	3.6	-
	vWA	43	1.7	0.5	2.6	3.0	2.8	-
	TPOX	1	1.4	-	1.4	1.4	1.4	-
	D18S51	8	1.4	0.3	2.0	2.3	1.9	-
	D5S818	1	1.7	-	1.7	1.7	1.7	-
	FGA	0	-	-	-	-	-	-

^aMaximum stutter percentage reported in the AmpFℓSTR^® Identifiler^® User Manual using the standard amplification protocol for 1187 population database DNA samples [12, 13]. ^bNumber of stutter peaks used for calculations. ^cStutter percentages for n-8 and n+4 positions were not reported using the Identifiler^® standard amplification protocol [12, 13]. SD: standard deviation.

ISSN: 2041-2223