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Table 2 Stutter percentages calculated for Identifiler STR loci amplified using the fast PCR protocol.

From: Development of a fast PCR protocol enabling rapid generation of AmpFℓSTR® Identifiler® profiles for genotyping of human DNA

   Fast-PCR protocol Applied Biosystems
Standard protocola
  Marker Nb Average
(%)
SD
(%)
Average + 2 SD
(%)
Average + 3 SD
(%)
Maximum
(%)
Maximum
(%)
n-4 D8S1179 165 7.4 1.4 10.1 11.5 12.5 8.2
  D21S11 188 8.5 1.3 10.9 12.2 13.1 9.4
  D7S820 168 5.5 1.8 8.8 10.6 11.8 8.2
  CSF1PO 151 6.0 1.4 8.7 10.1 12.6 9.2
  D3S1358 156 8.6 1.5 11.6 13.1 13.3 10.7
  THO1 174 2.5 0.9 4.2 5.1 4.9 5.1
  D13S317 164 4.8 2.1 9.2 11.3 12.4 8.0
  D16S539 160 6.6 1.7 10.2 11.9 11.1 10.4
  D2S1338 219 9.8 2.2 14.3 16.6 18.1 11.1
  D19S433 159 8.9 1.4 11.6 13.0 12.7 13.3
  vWA 176 8.4 2.4 13.6 16.0 21.1 12.6
  TPOX 179 3.6 1.4 6.0 7.3 9.8 4.8
  D18S51 198 9.2 2.7 14.5 17.2 18.1 17.0
  D5S818 145 7.3 1.2 9.7 10.9 11.1 6.8
  FGA 176 8.9 2.6 13.6 16.1 17.5 14.7
n+4 c D8S1179 73 0.8 0.3 1.3 1.6 1.6 -
  D21S11 61 1.3 0.7 2.5 3.2 4.2 -
  D7S820 18 1.0 0.6 2.0 2.6 2.9 -
  CSF1PO 36 1.0 0.6 2.0 2.6 3.6 -
  D3S1358 22 1.3 0.5 2.1 2.6 2.3 -
  THO1 6 1.2 0.9 2.7 3.6 3.0 -
  D13S317 22 1.0 0.5 1.9 2.4 2.4 -
  D16S539 18 1.2 0.7 2.3 3.0 3.2 -
  D2S1338 8 1.1 0.5 2.0 2.5 1.9 -
  D19S433 1 1.2 - 1.2 1.2 1.2 -
  vWA 21 1.7 0.6 2.9 3.5 2.9 -
  TPOX 17 1.9 0.7 3.5 4.2 3.1 -
  D18S51 13 1.7 0.7 2.8 3.4 3.2 -
  D5S818 9 2.0 1.6 4.6 6.2 5.0 -
  FGA 0 - - - - - -
n-8 c D8S1179 28 0.8 0.2 1.2 1.4 1.3 -
  D21S11 37 1.0 0.3 1.6 1.9 1.9 -
  D7S820 3 0.8 0.6 1.7 2.3 1.5 -
  CSF1PO 11 0.6 0.2 1.0 1.2 1.0 -
  D3S1358 16 1.2 0.3 1.7 2.0 1.9 -
  THO1 0 0.0 - - - - -
  D13S317 5 0.6 0.1 0.8 0.9 0.7 -
  D16S539 9 0.9 0.3 1.4 1.6 1.4 -
  D2S1338 55 1.4 0.4 2.3 2.7 2.3 -
  D19S433 16 1.4 0.7 2.5 3.2 3.6 -
  vWA 43 1.7 0.5 2.6 3.0 2.8 -
  TPOX 1 1.4 - 1.4 1.4 1.4 -
  D18S51 8 1.4 0.3 2.0 2.3 1.9 -
  D5S818 1 1.7 - 1.7 1.7 1.7 -
  FGA 0 - - - - - -
  1. aMaximum stutter percentage reported in the AmpFℓSTR® Identifiler® User Manual using the standard amplification protocol for 1187 population database DNA samples [12, 13]. bNumber of stutter peaks used for calculations. cStutter percentages for n-8 and n+4 positions were not reported using the Identifiler® standard amplification protocol [12, 13]. SD: standard deviation.
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