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Table 1 Differential DNA extraction protocols

From: Differential DNA extraction of challenging simulated sexual-assault samples: a Swiss collaborative study

Laboratories First cell lysis No. of washes Second cell lysis DNA Purification Concentration devices (laboratory number)
1, 3 Differexa; proteinase K 2 ATLb bufferc; DTTd; proteinase K QIAamp DNA mini kitc Microcone
2, 4, 7, 9 Lysis bufferf; proteinase K 1, 3, 4, 3 Lysis bufferf; DTTd; proteinase K Organic Precipitation (2,4,7); Centricone (9)
5 Chelexg; proteinase K 3 Chelexg; DTTd; proteinase K Chelexg Centricone
6 Lysis bufferf; proteinase K 3 DTTd; proteinase K QIAamp DNA mini kitc Centricone
8 ATLb bufferc; proteinase K 3 ATLb bufferc; DTTd; proteinase K QIAamp DNA micro kitc -
  1. aPromega AG, Dübendorf, Switzerland.
  2. bAnimal Tissue Lysis.
  3. cQiagen AG, Hombrechtikon, Switzerland.
  4. dDithiothreitol.
  5. eMillipore AG, Zug, Switzerland.
  6. fHome-made solution.
  7. gBio-Rad Laboratories AG, Reinach BL, Switzerland.