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Table 1 Differential DNA extraction protocols

From: Differential DNA extraction of challenging simulated sexual-assault samples: a Swiss collaborative study

Laboratories

First cell lysis

No. of washes

Second cell lysis

DNA Purification

Concentration devices (laboratory number)

1, 3

Differexa; proteinase K

2

ATLb bufferc; DTTd; proteinase K

QIAamp DNA mini kitc

Microcone

2, 4, 7, 9

Lysis bufferf; proteinase K

1, 3, 4, 3

Lysis bufferf; DTTd; proteinase K

Organic

Precipitation (2,4,7); Centricone (9)

5

Chelexg; proteinase K

3

Chelexg; DTTd; proteinase K

Chelexg

Centricone

6

Lysis bufferf; proteinase K

3

DTTd; proteinase K

QIAamp DNA mini kitc

Centricone

8

ATLb bufferc; proteinase K

3

ATLb bufferc; DTTd; proteinase K

QIAamp DNA micro kitc

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  1. aPromega AG, Dübendorf, Switzerland.
  2. bAnimal Tissue Lysis.
  3. cQiagen AG, Hombrechtikon, Switzerland.
  4. dDithiothreitol.
  5. eMillipore AG, Zug, Switzerland.
  6. fHome-made solution.
  7. gBio-Rad Laboratories AG, Reinach BL, Switzerland.