Development of a single base extension method to resolve Y chromosome haplogroups in sub-Saharan African populations

Table 4 Polymerase chain reaction (PCR) reagent mixtures for multiplex single base extension assays.

PCR reagent	YSNP1	Hg-A	Hg-B	Hg-B2b
	Concentration	Concentration	Concentration	Concentration
DNA	10 ng/μL	10 ng/μL	10 ng/μL	10 ng/μL
Buffer (10×)	1×	0.8×	1×	1×
MgCl₂ (25 mM)	2 mM	4 mM	3 mM	3 mM
dNTPs 2.5 mM)	300 μM	200 μM	200 μM	200 μM
Forward primer (10 μM)	See Table 1	See Table 1	See Table 1	See Table 1
Reverse primer (10 μM)	See Table 1	See Table 1	See Table 1	See Table 1
FastStart Taq (5 U/μL)	1 U	1 U	1 U	1 U
ddH₂O	Made up to 25 μL	Made up to 25 μL	Made up to 25 μL	Made up to 25 μL
	Hg-E	Hg-E1b1a	Hg-E1b1b1
PCR reagent	Concentration	Concentration	Concentration
DNA	10 ng/μL	10 ng/μL	10 ng/μL
Buffer (10×)	1.25×	1.25×	1.25×
MgCl₂ (25 mM	3 mM	3 mM	3 mM
dNTPs (2.5 mM)	200 μM	200 μM	200 μM
Forward primer (10 μM)	See Table 1	See Table 1	See Table 1
Reverse primer (10 μM)	See Table 1	See Table 1	See Table 1
FastStart Taq (5 U/μL)	1 U	1 U	1 U
ddH₂O	Made up to 25 μL	Made up to 25 μL	Made up to 25 μL