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Table 4 Polymerase chain reaction (PCR) reagent mixtures for multiplex single base extension assays.
| PCR reagent | YSNP1 | Hg-A | Hg-B | Hg-B2b |
|---|---|---|---|---|
| Concentration | Concentration | Concentration | Concentration | |
| DNA | 10 ng/μL | 10 ng/μL | 10 ng/μL | 10 ng/μL |
| Buffer (10×) | 1× | 0.8× | 1× | 1× |
| MgCl2 (25 mM) | 2 mM | 4 mM | 3 mM | 3 mM |
| dNTPs 2.5 mM) | 300 μM | 200 μM | 200 μM | 200 μM |
| Forward primer (10 μM) | See Table 1 | See Table 1 | See Table 1 | See Table 1 |
| Reverse primer (10 μM) | See Table 1 | See Table 1 | See Table 1 | See Table 1 |
| FastStart Taq (5 U/μL) | 1 U | 1 U | 1 U | 1 U |
| ddH2O | Made up to 25 μL | Made up to 25 μL | Made up to 25 μL | Made up to 25 μL |
| Hg-E | Hg-E1b1a | Hg-E1b1b1 | ||
| PCR reagent | Concentration | Concentration | Concentration | |
| DNA | 10 ng/μL | 10 ng/μL | 10 ng/μL | |
| Buffer (10×) | 1.25× | 1.25× | 1.25× | |
| MgCl2 (25 mM | 3 mM | 3 mM | 3 mM | |
| dNTPs (2.5 mM) | 200 μM | 200 μM | 200 μM | |
| Forward primer (10 μM) | See Table 1 | See Table 1 | See Table 1 | |
| Reverse primer (10 μM) | See Table 1 | See Table 1 | See Table 1 | |
| FastStart Taq (5 U/μL) | 1 U | 1 U | 1 U | |
| ddH2O | Made up to 25 μL | Made up to 25 μL | Made up to 25 μL |
