Table 4 Polymerase chain reaction (PCR) reagent mixtures for multiplex single base extension assays.
PCR reagent | YSNP1 | Hg-A | Hg-B | Hg-B2b |
|---|---|---|---|---|
| Â | Concentration | Concentration | Concentration | Concentration |
DNA | 10 ng/μL | 10 ng/μL | 10 ng/μL | 10 ng/μL |
Buffer (10×) | 1× | 0.8× | 1× | 1× |
MgCl2 (25 mM) | 2 mM | 4 mM | 3 mM | 3 mM |
dNTPs 2.5 mM) | 300 μM | 200 μM | 200 μM | 200 μM |
Forward primer (10 μM) | See Table 1 | See Table 1 | See Table 1 | See Table 1 |
Reverse primer (10 μM) | See Table 1 | See Table 1 | See Table 1 | See Table 1 |
FastStart Taq (5 U/μL) | 1 U | 1 U | 1 U | 1 U |
ddH2O | Made up to 25 μL | Made up to 25 μL | Made up to 25 μL | Made up to 25 μL |
| Â | Hg-E | Hg-E1b1a | Hg-E1b1b1 | Â |
PCR reagent | Concentration | Concentration | Concentration | Â |
DNA | 10 ng/μL | 10 ng/μL | 10 ng/μL |  |
Buffer (10×) | 1.25× | 1.25× | 1.25× |  |
MgCl2 (25 mM | 3 mM | 3 mM | 3 mM | Â |
dNTPs (2.5 mM) | 200 μM | 200 μM | 200 μM |  |
Forward primer (10 μM) | See Table 1 | See Table 1 | See Table 1 |  |
Reverse primer (10 μM) | See Table 1 | See Table 1 | See Table 1 |  |
FastStart Taq (5 U/μL) | 1 U | 1 U | 1 U |  |
ddH2O | Made up to 25 μL | Made up to 25 μL | Made up to 25 μL |  |