PCR reagent
|
YSNP1
|
Hg-A
|
Hg-B
|
Hg-B2b
|
---|
|
Concentration
|
Concentration
|
Concentration
|
Concentration
|
---|
DNA
|
10 ng/μL
|
10 ng/μL
|
10 ng/μL
|
10 ng/μL
|
Buffer (10×)
|
1×
|
0.8×
|
1×
|
1×
|
MgCl2 (25 mM)
|
2 mM
|
4 mM
|
3 mM
|
3 mM
|
dNTPs 2.5 mM)
|
300 μM
|
200 μM
|
200 μM
|
200 μM
|
Forward primer (10 μM)
|
See Table 1
|
See Table 1
|
See Table 1
|
See Table 1
|
Reverse primer (10 μM)
|
See Table 1
|
See Table 1
|
See Table 1
|
See Table 1
|
FastStart Taq (5 U/μL)
|
1 U
|
1 U
|
1 U
|
1 U
|
ddH2O
|
Made up to 25 μL
|
Made up to 25 μL
|
Made up to 25 μL
|
Made up to 25 μL
|
|
Hg-E
|
Hg-E1b1a
|
Hg-E1b1b1
| |
PCR reagent
|
Concentration
|
Concentration
|
Concentration
| |
DNA
|
10 ng/μL
|
10 ng/μL
|
10 ng/μL
| |
Buffer (10×)
|
1.25×
|
1.25×
|
1.25×
| |
MgCl2 (25 mM
|
3 mM
|
3 mM
|
3 mM
| |
dNTPs (2.5 mM)
|
200 μM
|
200 μM
|
200 μM
| |
Forward primer (10 μM)
|
See Table 1
|
See Table 1
|
See Table 1
| |
Reverse primer (10 μM)
|
See Table 1
|
See Table 1
|
See Table 1
| |
FastStart Taq (5 U/μL)
|
1 U
|
1 U
|
1 U
| |
ddH2O
|
Made up to 25 μL
|
Made up to 25 μL
|
Made up to 25 μL
| |
- dNTP, deoxyribonucleotide triphosphate; ddH20, double distilled water