Accurate, rapid and high-throughput detection of strain-specific polymorphisms in Bacillus anthracis and Yersinia pestis by next-generation sequencing

Table 1 B. anthracis SOLiD™ system sequencing statistics

	A0032 (China)	A0324 (Slovakia)	A0377 (Haiti)	A2012 (Florida Ames)
Beads found, n	25,789,050	3,849,205	5,393,062	19,073,351
Uniquely placed beads (≤ 3 mismatches), n (%)	15,561,852 (60.34)	2,803,450 (72.83)	4,020,634 (74.55)	13,447,970 (70.51)
Bases not uniquely covered, n (%)	74,268 (1.35)	95,241 (1.73)	78,849 (1.43)	68,629 (1.25)
Chromosome median coverage^a	93×	16×	23×	79×
pXO1 median coverage	165×	34×	66×	241×
pXO1 mean copy number, n	1.8	2.1	2.9	3.1
pXO2 median coverage	135×	26×	42×	124×
pXO2 mean copy number	1.5	1.6	1.8	1.6

ISSN: 2041-2223