Figure 2

PCR amplification of decayed and degraded DNA. (A) Ssp2201 Atlantic salmon-specific microsatellite primers used on ensilage sample. Samples were separated by electrophoresis in 2% agarose gels. DNA marker 50 bp ladder (lane 1), ensilage samples (lane 2-5), high-quality genomic DNA extracted from fresh salmon (lane 6), no template (lane 7). (B) Fish primers used on canned products. Canned salmon fillet (lane 1-2), canned salmon pâté (lane 3-4), no template (lane 5), high-quality genomic DNA extracted from fresh salmon (lane 6), DNA marker 50 bp ladder (lane 7). (C) SsaCOI030 primers used on canned products. Canned salmon fillet (lane 1-2), canned salmon pâté (lane 3-4), high-quality genomic DNA extracted from fresh salmon (lane 5), no template (lane 6), DNA marker 50 bp ladder (lane 7). (D) SsaCOI030 primers used on seawater decayed Atlantic salmon and rainbow trout fillet and ensilage. Atlantic salmon fillet collected on days 3, 5, 7, 10, 13, 17, 21, 24, 28 and 31 (lane 1, 3, 5, 7, 9, 11, 13, 15), rainbow trout fillet sampled on the same days as Atlantic salmon (lane 2, 4, 6, 8, 10, 12, 14, 16), DNA marker 50 bp ladder (lane 17), ensilage (lane 18-21), no template (lane 22), high-quality genomic DNA extracted from fresh salmon (lane 23).