From: Next-generation sequencing technologies and applications for human genetic history and forensics
Instrument | Read length (nucleotides) | No. of readsa | Output (Gb)a | No. of samplesa, b | Runtime | Advantages | Disadvantages |
---|---|---|---|---|---|---|---|
Roche 454 GS FLX+ | 700c | 1 × 106 | 0.7 | 192d | 23 h | Long reads, short run time | Homopolymer errors, expensive |
Illumina HiSeq2000 | 100e | 3 × 109 | 600 | 384 | 11 daysf | High yield | No. of index tags limiting |
Life Technologies SOLiD 5500xl | 75g | 1.5 × 109 | 180 | 1,152 | 14 daysf | Inherent error correction | Short readsg |
Roche 454 GS Junior | 400c | 1 × 105 | 0.035 | 132 | 9 h | Long reads | Homopolymer errors, expensive |
Illumina MiSeq | 150 | 5 × 106 | 1.5 | 96 | 27 h | Short run time, ease of use | Expensive per base |
Ion Torrent PGM Ion 316 chip | > 100h | 1 × 106 | 0.1 | 16 | 2 h | Short run time, low reagent cost | Not well evaluated |
Helicos BioSciences HeliScope | 35h | 1 × 109 | 35 | 4,800 | 8 days | SMS, sequences RNA | Short reads, high error rate |
Pacific Biosciences PacBio RS | > 1,000h | 1 × 105 | 0.1 | 1 | 90 min | SMS, long reads, short run time | High error rate, low yield |