Table 1 Primer pairs used to amplify the seven overlapping fragments of HVS-I with molecular details
Amplicon size (bp) | Primer name | Primer range | Primer sequence 5′-3′ | Sequence information range (bp) | Reference |
|---|---|---|---|---|---|
170 | L15422 H15555 | 15404-15422 15555-15574 | CTCTTGCTCCACCATCAGC TTATATGCATGGGGCAAACC | 133 | [124] |
219 | L15511 H15692 | 15490-15511 15692-15709 | ACTGTGCTATGTCAGTATCTCC TTGATGGTTTCTCGAGGC | 181 | [124] |
246 | L15574 H15780 | 15555-15574 15780-15801 | GGTTTGCCCCATGCATATAA AAGTAAGAACCAGATGCCAGGT | 206 | [126] |
148 | L15764 H15873 | 15744-15764 15873-15892 | CCCATACTAACGTGGGGGTTA TGTGTGATCATGGGCTGATT | 109 | [126] |
129 | L15857 H15945 | 15836-15857 15945-15965 | ATTCTCGCAAATGGGACATC GCGGTCGTAGGTGAGTGATAG | 88 | [126] |
137 | L15892 H15991 | 15873-15892 15991-16010 | AATCAGCCCATGATCACACA GGCATATTCCCTGAGAGCAG | 99 | [126] |
154 | L15982 H16097 | 15963-15982 16097-16117 | CGCAACGGCACTAACTCTAA TGTCCTGAAACCATTGACTGA | 115 | [126] |