Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations

Table 2 VNTR primers used in MLVA typing of Pseudomonas syringae pathovar tomato DC3000

VNTR locus primer^a	Direction	Primer sequence (5′ → 3′)	Product size, bp
715-F	Forward	TGTGCGATGACACGCTTACCCATA	314
715-R	Reverse	TATTCGCGGACATTCGTGACAAGA	314
1570-F	Forward	AGTCTCTGCTCTTTGGTTGGCGTA	216
1570-R	Reverse	GTCTGATGTACATGGTGCGCTGGT	216
1929-F	Forward	CGAACAGAACGCGGCCTTCAAATA	511
1929-R	Reverse	ACAGCGACTGAGCTGATTCAGGAT	511
337-F	Forward	TGGAGCACAAACTGCTCTGAGTCT	440
337-R	Reverse	TACAGAGATGGCGCGATTGAGCA	440
919/920-F	Forward	AAACATCAGCCAGCAAATCACCCG	829
919/920-R	Reverse	AACTGTTATGCCTTGTCGCACAGC	829

MLVA, Multi-locus variable number tandem repeat analysis; VNTR, Variable number tandem repeat.
^aFrom: Baker[12].

ISSN: 2041-2223