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Table 2 Centrifugal filter purification of mock crime scene DNA extracts using Amicon Ultra 30 K and Microsep 30 K

From: Purification of crime scene DNA extracts using centrifugal filter devices

Sample type DNA purification Average DNA concentration (ng/μL) with CV (%) Detected STR alleles (>50 rfu) Average of total sum of STR peak heights (rfu)awith CV (%) Average of intra-locus balance (0 to 1)b Average of inter-loci balance (0 to 1)c
Blood on denim (n = 3) None 0.46 (27%) 96% 30,776 (20%) 0.83 0.90
  Microsep 30 K 0.22(12%) 100% 42,113 (10%) 0.87 0.96
  Amicon Ultra 30 K 0.27(15%) 96% 51,596 (38%) 0.84 0.95
Blood on kitchen paper (n = 10) None 0.32 (79%) N/A N/A N/A N/A
  Microsep 30 K 0.23 (83%) 100% 20,230 (78%) 0.89 0.99
  Amicon Ultra 30 K 0.23 (85%) 100% 21,115 (96%) 0.90 0.99
Hair (n = 10) None 0.66 (77%) N/A N/A N/A N/A
  Microsep 30 K 0.39 (77%) 98% 32,135 (77%) 0.88 0.98
  Amicon Ultra 30 K 0.72 (55%) 100% 62,670 (56%) 0.93 0.99
Rape case samples, semen fraction (n = 24) None 1.10 (34%) N/A N/A N/A N/A
  Microsep 30 K 0.56 (43%) 100% 57,088 (37%) 0.89 0.98
  Amicon Ultra 30 K 0.86 (25%) 100% 85,441 (23%) 0.91 0.99
Rape case samples, epithelial fraction (n = 24) None 1.09 (51%) N/A N/A N/A N/A
  Microsep 30 K 0.68 (39%) 100% 33,498 (47%) 0.87 0.97
  Amicon Ultra 30 K 0.89 (30%) 100% 42,508 (30%) 0.88 0.97
Saliva on envelope (n = 3) None 0.083 (22%) 100% 53,754 (14%) 0.90 0.98
  Microsep 30 K 0.050 (8%) 100% 43,682 (10%) 0.91 0.99
  Amicon Ultra 30 K 0.054 (17%) 100% 63,168 (6%) 0.88 0.99
Touch stains (mini-tape) (n = 10) None 0.023 (71%) N/A N/A N/A N/A
  Microsep 30 K 0.027 (55%) 95% 11,281 (73%) 0.74 0.96
  Amicon Ultra 30 K 0.024 (59%) 97% 12,207 (77%) 0.76 0.96
  1. aTotal sum of peak heights have been normalized against the sample volume in PCR. bMean local balance, that is, the mean of the heterozygote balances in all markers of the electropherograms, was used for calculation of intra-locus balance. cNormalized Shannon entropy [32, 33] was used to calculate inter-locus balance. CV, Coefficient of variation; rfu, Relative fluorescence units.