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Table 1 HIrisPlex genotyping results together with sample age and starting DNA amount for the 26 samples tested

From: Bona fide colour: DNA prediction of human eye and hair colour from ancient and contemporary skeletal remains

Sample ID Sample/postmortem age Starting template DNA (ng) Genotype
    1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
S1 T/CR 0.6 C G C C G/T C C G C G T A G A C/A C/T G G A C/T T T C G/T
S2 T/CR 1.7 C G C/T C G C C G C G T A G A C/A C G G A C/T T T C/T G/T
S3 T/CR 1.2 C G C/T C G C C G C G T A G G/A C/A C/T G/T G G C/T T G C/T G/T
S4 T/C 0.3 - G C C/T G C C G C G T A G A C/A C G/T G A C T G/T C T
S5 B/C Ua C G C C G C C G C G T C C A C/A C/T G/T G A C T T C G
S6 B/C 0.3 C G C C G C C G C G T A G A C C G/T G A T T G/T C T
S7 B/C 0.15 - G C C G C C G C G T C C A C/A C/T G/T G A C T T C G
S8 B/C U C G C C G C C G C G T C/A G/C A C/A C G G A C/T T G C G/T
S9 B/C 0.5 C G C C G C C G C G T C/A G/C A C/A C/T G G A C T T C/T G/T
S10 B/C 0.1 C G C C G C C G C G T C/A G/C A C C G G G/A T T G/T C/T G/T
S11 T/2WW 0.6 C G C C/T G C C G C G T A G A A C G/T G A C T T C T
S12 T/2WW 2 C G C C/T G C C G C G C/T A G A C C G G A C T G/T C/T G/T
S13 T/2WW 0.3 C G C C/T G C C G C G T A G A A C G G A C/T T T C G
S14 T/2WW 0.2 C G C C G C C G C G T A G A C/A C T G G/A C T G C/T T
S15 T/2WW 1.2 C G C C G C C G C G T A G A A C G/T G G/A C T G C G
S16 T/2WW 0.1 C G C C G C C G C G T A G A C/A C G G A C T T C T
S17 T/2WW 0.8 C G C C G C C G C G T A G A C/A C G G A C/T T G/T C/T G/T
S18 T/2WW 2.8 C G C C/T G C C G C G T A G A C/A C G/T G A C C/T G/T C/T G/T
S19 T/2WW 0.8 C G C C G C C G C G T A G A A C G G A C T T C T
S20 T/2WW 0.4 C G C C G C C G C G T A G G/A C C G/T G A C/T T G/T C/T G/T
S21 T/2WW 0.3 C G C C G C C G C G T A G A C/A C G G A C T G/T C/T T
S22 T/2WW 0.7 C G C C G C C G C G T A G G A C G G G C C/T G C T
S23 T/2WW 1.3 C G C C G C C G C G T A G A C/A C G/T G A C/T T G/T C T
S24 T/XII 0.03b C G C C G C C G C G/A T A G A C/A C G G A C/T T G/T T G/T
S25 T/XIV 0.01b - G C C - C T G C - T A G A C/A C T G G C T G C/T G/T
S26 T/XIV 0.16 C G C C G C C G C G/A T A G A C/A C T G A C T G/T C G/T
  1. a U - DNA concentration was not determined due to complete inhibiton of Quantifiler™ Human DNA Quantification Kit reaction.
  2. b DNA concentration may be higher as significant inhibition of Quantifiler™ Human DNA Quantification Kit reaction was observed. DNA markers are shown in the following order from 1 to 24: 1-N29insA, 2-rs11547464, 3-rs885479, 4-rs1805008, 5-rs1805005, 6-rs1805006, 7-rs1805007, 8-rs1805009, 9-Y152OCH, 10-rs2228479, 11-rs1110400, 12-rs28777, 13- rs16891982, 14- rs12821256, 15-rs4959270, 16- rs12203592, 17- rs1042602, 18-rs1800407, 19-rs2402130, 20-rs12913832, 21- rs2378249, 22-rs12896399, 23-rs1393350, 24- rs683. Samples S5 and S7 come from the same individual. In the column Sample/postmortem age: T indicates tooth and B indicates bone. CR-indicates contemporary and relatively fresh specimen (one to two years postmortem age); C-indicates contemporary but older than CR; 2WW-indicates samples from the World War 2; XII and XIV-indicate centuries assumed from historical evidence.
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