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Table 3 Ion Torrent PGM whole mitochondrial DNA sequencing data, after one and two rounds of enrichment

From: DNA capture and next-generation sequencing can recover whole mitochondrial genomes from highly degraded samples for human identification

ACAD ID Rounds capture Percentage coverage Average length (± SD) Number of bases covered Unique reads Mean X coverage (± SD) Haplotype
4464B 1 97.4% 42 (± 11.0) 16,136 7,069 18 (± 12.3) HV0e
4464B (ancient) 2 99.60% 45 (± 11.7) 16,507 14,837 40 (± 19.8) HV0e
10730A 1 96.6% 43 (± 11.3) 16,004 5,760 15 (± 10.7) B2b
10730A (ancient) 2 99.80% 47 (± 12.2) 16,540 16,138 46 (± 20.2) B2b
11995A 1 62.3% 40 (± 10.2) 10,321 553 1 (± 1.6) Low coverage
11995A (forensic) 2 97.70% 45 (± 12.9) 16,188 6,012 16 (± 11.2) H1a
Extraction blank 1 1.5% 41 (± 18.4) 248 6 0 n/a
Extraction blank (EBC11049) 2 0.20% 0 28 1 0 n/a
8727C (forensic) 2 99.50% 43 (± 9.3) 16,487 7,756 20 (± 9.7) U5a2a1fNEW
9210A (forensic) 2 100% 65 (± 20.4) 16,569 7,865 31 (± 14.1) J1c12
  1. ACAD, Australian Centre for Ancient DNA; n/a, non-applicable.