Skip to main content

Table 3 Ion Torrent PGM whole mitochondrial DNA sequencing data, after one and two rounds of enrichment

From: DNA capture and next-generation sequencing can recover whole mitochondrial genomes from highly degraded samples for human identification

ACAD ID

Rounds capture

Percentage coverage

Average length (± SD)

Number of bases covered

Unique reads

Mean X coverage (± SD)

Haplotype

4464B

1

97.4%

42 (± 11.0)

16,136

7,069

18 (± 12.3)

HV0e

4464B (ancient)

2

99.60%

45 (± 11.7)

16,507

14,837

40 (± 19.8)

HV0e

10730A

1

96.6%

43 (± 11.3)

16,004

5,760

15 (± 10.7)

B2b

10730A (ancient)

2

99.80%

47 (± 12.2)

16,540

16,138

46 (± 20.2)

B2b

11995A

1

62.3%

40 (± 10.2)

10,321

553

1 (± 1.6)

Low coverage

11995A (forensic)

2

97.70%

45 (± 12.9)

16,188

6,012

16 (± 11.2)

H1a

Extraction blank

1

1.5%

41 (± 18.4)

248

6

0

n/a

Extraction blank (EBC11049)

2

0.20%

0

28

1

0

n/a

8727C (forensic)

2

99.50%

43 (± 9.3)

16,487

7,756

20 (± 9.7)

U5a2a1fNEW

9210A (forensic)

2

100%

65 (± 20.4)

16,569

7,865

31 (± 14.1)

J1c12

  1. ACAD, Australian Centre for Ancient DNA; n/a, non-applicable.