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Table 2 Mitochondrial and nuclear quantitative PCR (qPCR) and STR typing result

From: DNA capture and next-generation sequencing can recover whole mitochondrial genomes from highly degraded samples for human identification

Sample ID Mitochondrial DNA Nuclear DNA
  qPCR qPCR qPCR STR typing (Profiler Plus kit, 10 loci) 34 cycles
  77 bp target (copies/μL) 67 bp target (copies/μL) 67 bp target (ng/μL)    
     Number of loci Percentage genotyping success None, partial or full profile obtained
4464B 15,727 <1 copy (0.54) 0.001794 8 80% Partial
10730A 62,592 0 0 Amel only 10% Partial
8727C 350 <1 copy (0.025) 0.000082 3 30% Partial
11995A 2,715 <1 copy (0.0091) 0.00003 2 20% Partial
9210A n/a n/a n/a n/a n/a n/a
Negative control (H20) 0 <1 copy (0.034) 0.000111 0 0% None
Female positive control 7,896,895 543 1.79213 10 100% Full
  1. n/a, non-applicable (The sample was excluded from testing due to the low quantity of DNA extract. Quantitative PCR data is presented as a geometric average using triplicate values.).