Figure 2

Autofluorescence of cyclic olefin polymer and glass by excitation at 488 nm and measured across the visible wavelength spectrum between 470 and 700 nm. The peak at about 570nm is the Raman emission peak of cyclic olefin polymer. The S&D biochip is fabricated from cyclic olefin polymer, allowing for low autofluorescence in a single-use component. By contrast, glass separation capillaries and biochips are typically reused, requiring more complicated instrumentation and leading to potential cross contamination.