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Table 1 Locus-specific peak height intensity and heterozygote peak height ratios for 133 Identifiler profiles generated using the fast PCR protocol.

From: Development of a fast PCR protocol enabling rapid generation of AmpFℓSTR® Identifiler® profiles for genotyping of human DNA

Marker Average peak height (RFU) Inter-loci balance a Average heterozygote
peak height ratio b
D8S1179 2726 ± 1103 1.90 0.87 ± 0.08
D21S11 1095 ± 539 0.78 0.86 ± 0.09
D7S820 870 ± 471 0.62 0.86 ± 0.09
CSF1PO 885 ± 493 0.63 0.86 ± 0.11
D3S1358 1745 ± 683 1.23 0.88 ± 0.09
THO1 1615 ± 753 1.14 0.87 ± 0.09
D13S317 1344 ± 595 0.95 0.86 ± 0.09
D16S539 1024 ± 498 0.73 0.84 ± 0.10
D2S1338 1038 ± 572 0.74 0.83 ± 0.11
D19S433 1750 ± 701 1.21 0.88 ± 0.08
vWA 1685 ± 712 1.19 0.83 ± 0.10
TPOX 1518 ± 698 1.07 0.88 ± 0.08
D18S51 959 ± 513 0.68 0.82 ± 0.10
D5S818 1604 ± 625 1.14 0.88 ± 0.09
FGA 575 ± 238 0.41 0.86 ± 0.09
  1. aThe inter-loci balance was assessed by dividing the average peak height at one locus by the average peak height at all loci. Ideal value is 1.0. bNumber of allele pairs used for the heterozygote peak height ratio calculations varied between 89 and 126. RFU: relative fluorescence units.
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