Development of a fast PCR protocol enabling rapid generation of AmpFℓSTR® Identifiler® profiles for genotyping of human DNA

Table 1 Locus-specific peak height intensity and heterozygote peak height ratios for 133 Identifiler profiles generated using the fast PCR protocol.

Marker	Average peak height (RFU)	Inter-loci balance ^a	Average heterozygote peak height ratio ^b
D8S1179	2726 ± 1103	1.90	0.87 ± 0.08
D21S11	1095 ± 539	0.78	0.86 ± 0.09
D7S820	870 ± 471	0.62	0.86 ± 0.09
CSF1PO	885 ± 493	0.63	0.86 ± 0.11
D3S1358	1745 ± 683	1.23	0.88 ± 0.09
THO1	1615 ± 753	1.14	0.87 ± 0.09
D13S317	1344 ± 595	0.95	0.86 ± 0.09
D16S539	1024 ± 498	0.73	0.84 ± 0.10
D2S1338	1038 ± 572	0.74	0.83 ± 0.11
D19S433	1750 ± 701	1.21	0.88 ± 0.08
vWA	1685 ± 712	1.19	0.83 ± 0.10
TPOX	1518 ± 698	1.07	0.88 ± 0.08
D18S51	959 ± 513	0.68	0.82 ± 0.10
D5S818	1604 ± 625	1.14	0.88 ± 0.09
FGA	575 ± 238	0.41	0.86 ± 0.09

^aThe inter-loci balance was assessed by dividing the average peak height at one locus by the average peak height at all loci. Ideal value is 1.0. ^bNumber of allele pairs used for the heterozygote peak height ratio calculations varied between 89 and 126. RFU: relative fluorescence units.

ISSN: 2041-2223