Skip to main content
Figure 2 | Investigative Genetics

Figure 2

From: Development of a fast PCR protocol enabling rapid generation of AmpFℓSTR® Identifiler® profiles for genotyping of human DNA

Figure 2

Representative Identifiler profiles from forensically relevant samples amplified using the optimized fast PCR protocol. Reactions were conducted in a 15 μL volume containing 1X SpeedSTAR™ Fast Buffer I, 1.0 U SpeedSTAR™ HS DNA polymerase, 350 μM dNTPs, 3.0 μL Identifiler primer set and 1.0 ng of DNA from various sample types (indicated). Amplification was carried out as follows: 95°C for 1 min; 95°C for 5 s, 61°C for 15 s for 28 cycles; and 72°C for 1 min.

Back to article page

Investigative Genetics

ISSN: 2041-2223

Contact us