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Figure 1 | Investigative Genetics

Figure 1

From: Development of a fast PCR protocol enabling rapid generation of AmpFℓSTR® Identifiler® profiles for genotyping of human DNA

Figure 1

Identifiler profiles generated across a range of annealing temperatures during optimization of the fast PCR protocol. Reactions were conducted in a 15 μL volume containing 1X SpeedSTAR™ Fast Buffer I, 1 U SpeedSTAR™ HS DNA polymerase, 350 μM dNTPs, 3.0 μL Identifiler primer set and 1.0 ng of DNA from the control cell line GM9948. Amplification was carried out as follows: 95°C for 1 min; 95°C for 5 s, 59°C to 64°C for 15 s for 28 cycles; and 72°C for 1 min. A profile generated using the standard Identifiler amplification condition on the GeneAmp® PCR System 9700 thermal cycler is included for comparison.

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