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Figure 3 | Investigative Genetics

Figure 3

From: Isolating DNA from sexual assault cases: a comparison of standard methods with a nuclease-based approach

Figure 3

Casework evidence from rape victims processed with the standard differential lysis or Erase. Case 1: (A) victim’s profile obtained from a buccal swab, (B) suspect’s profile obtained from a buccal swab, (C) NGM SElect (Applied Biosystems, Carlsbad, CA, USA) profile of the sperm fraction DNA isolated using the standard method, and (D) SEfiler (Applied Biosystems) profile of the sperm fraction DNA isolated using Erase. Note that the loci in (C) and (D) are the same (amelogenin, D8S1179, D21S11, D18S51) but were obtained using different kits (NGM Select or SEfiler). Case 2: (E) profile of the sperm fraction DNA isolated using the standard method, and (F) profile of the sperm fraction DNA isolated using Erase. The correct female genotype for Case 2 as determined from a buccal swab is: D8S1179 (13,14), SE33 (24.2,25.2).

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