Table 4 Peak heights
Homozygous Peaks (RFU) | Heterozygous Peaks (RFU) | |||||||
|---|---|---|---|---|---|---|---|---|
n | Range | Mean | Std Dev. | n | Range | Mean | Std Dev. | |
100 pg Starting Template | ||||||||
30 PCR Cycle Amplification | ||||||||
Non-split Samples | 57 | 185 to 847 | 520 | 170 | 360 | 55 to 725 | 261 | 116 |
34 PCR Cycle Amplification | ||||||||
Non-split Samples | 57 | 662 to 7,609 | 4,129 | 1,767 | 366 | 139 to 5,805 | 1,925 | 1,233 |
Split Samples | 168 | 166 to 6,131 | 1,610 | 1,090 | 949 | 51 to 4,975 | 763 | 638 |
25 pg Starting Template | ||||||||
30 PCR Cycle Amplification | ||||||||
Non-split Samples | 49 | 51 to 337 | 149 | 73 | 200 | 50 to 360 | 110 | 56 |
34 PCR Cycle Amplification | ||||||||
Non-split Samples | 53 | 212 to 3,475 | 1,259 | 805 | 287 | 58 to 3,500 | 708 | 602 |
Split Samples | 122 | 59 to 2,144 | 541 | 369 | 458 | 53 to 2,074 | 386 | 260 |