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Table 1 Allele drop out (ADO)

From: Comparison of STR profiling from low template DNA extracts with and without the consensus profiling method

 

Number of ADO

% Heterozygote Loci with ADO

100 pg Starting Template

30 PCR Cycle Amplification

Non-split Samples (na = 183)

6

3%

34 PCR Cycle Amplification

Non-split Samples (n = 183)

0

0%

Split Samples (n = 549)

114

21%

Consensus Profiles (n = 183)

16

9%

25 pg Starting Template

30 PCR Cycle Amplification

Non-split Samples (n = 183)

80

44%

34 PCR Cycle Amplification

Non-split Samples (n = 183)

61

33%

Split Samples (n = 549)

250

46%

Consensus Profiles (n = 183)

92

50%

  1. a n is the total number of heterozygote loci.
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Investigative Genetics

ISSN: 2041-2223

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