Method: a single nucleotide polymorphism genotyping method for Wheat streak mosaic virus

Investigative Genetics

Table 1 SNaPshot primers designed for WSMV

Name^a	Length (nt)	Sequence (5′-3′)^b	T_M	Strand^c	Final Conc. (μM)
30 C	30	GACTGGCGTGTTCTCCCTCACATCATCTGC	65.1	F	0.1
35 C	35	GACTGACTGACAACTGAACAACTCAACACCTGGAT	63.5	R	0.2
40 C	40	GACTGACTGACTGACTGGCATATCTGTTGTCGATAAGTTC	63.5	F	0.1
45 C	45	GACTGACTGACTGACTGACTGTAGTTTCTACTGTGCTCACGCAAG	66.5	F	0.2
50 C	50	GACTGACTGACTGACTGACTGACTTTCGCAGCTTTGTACATCGGTTCAAT	67.6	F	0.4
55 C	55	GACTGACTGACTGACTGACTGACTGACTGATCTCTGGGCACGTTGTGTTGATTAT	72.05	F	0.4
60 C	60	GACTGACTGACTGACTGACTGACTGACTGACTGACCCCTTGGTATGATAGGTTTTCCAAT	72.82	F	0.4
65 C	65	GACTGACTGACTGACTGACTGACTGACTGACTGACTGACTTGTTCTGTATTCCGCGTAGCCTGTT	74.74	F	0.4
30 H	30	GACTGCAAGTTGTCTTAGCACATCACCAAA	60.8	F	0.4
35 H	35	GACTGACTGACTGACTGCGCAAGTTACCTGGAAGC	66.3	R	0.4
40 H	40	GACTGACTGACTGACTGCGTATCCAGACAGCGATGTAACA	66.4	R	0.4
45 H	45	GACTGACTGACTGACTGACTGAATTGTTCCATCTTCTAGCATCTT	64.7	F	0.1
50 H	50	GACTGACTGACTGACTGACTGACTGACTCGAATTTTAGGTTCGTTGATTT	65.8	R	0.1
55 H	55	GACTGACTGACTGACTGACTGACTGACTGACTTTAGACTCGCCGTTAATTTCCAT	71.31	F	0,4
60 H	60	GACTGACTGACTGACTGACTGACTGACTGACTGACTGATTTGTCACTGGGAAGGAAATGT	72.82	R	0.2

^aThe letter of the primer name indicates the target is either the coat protein (C) or the helper-component protease (H) segment of the genome.
^bEach primer contains a segment that anneals to the template (underlined) and a GACT tail to adjust the length of the primer.
^cThe primer anneals to either the forward (F) or reverse (R) strand. nt, nucleotide; WSMV, Wheat streak mosaic virus.

ISSN: 2041-2223