Gel electrophoresis of CP and HCPro amplification products from WSMV near-neighbors. Lanes 2–8 and 11–17 are ONMV, WSSMV, MDMV, HPV, CYDV-RPV, BYDV-PAV, and healthy wheat PCR products for CP and HC-Pro assays, respectively. Lanes 1 and 20 are a 100 bp DNA ladder. Lanes 9 and 18 are the positive control CP and HC-PRO WSMV plasmid, respectively. Lanes 10 and 19 are negative controls. Only the positive controls produced approximately 1,300 bp amplicons, corresponding to WSMV. BYDV-PAV, Barley yellow dwarf virus PAV; CP, coat protein; CYDV-RPV, Cereal yellow dwarf virus RPV; HC-Pro, helper-component; HPV, High plains virus; MDMV, Maize dwarf mosaic virus; ONMV, Oat necrotic mottle virus; PCR, polymerase chain reaction; WSMV, Wheat streak mosaic virus; WSSMV, Wheat spindle streak mosaic virus.