Figure 1From: Method: a single nucleotide polymorphism genotyping method for Wheat streak mosaic virusWSMV CP multiplex electropherogram. The height of each peak indicates the concentration of fluorescent units and the location of the peaks along the x-axis indicates the size of the fragment in nucleotides; left to right showing smallest to largest. A) Amplification of synthetic targets using eight SNP-specific primers for the WSMV CP, showing a single amplified target for each primer. B) Negative control (using sterile nanopure water instead of the synthetic templates) for the CP amplification demonstrating the absence of non-target amplification. The peaks present (labeled according to fragment size) are size standards to help with the assignment of the fragment sizes. CP, coat protein; SNP, single nucleotide polymorphism; WSMV, Wheat streak mosaic virus.Back to article page