WSMV CP multiplex electropherogram. The height of each peak indicates the concentration of fluorescent units and the location of the peaks along the x-axis indicates the size of the fragment in nucleotides; left to right showing smallest to largest. A) Amplification of synthetic targets using eight SNP-specific primers for the WSMV CP, showing a single amplified target for each primer. B) Negative control (using sterile nanopure water instead of the synthetic templates) for the CP amplification demonstrating the absence of non-target amplification. The peaks present (labeled according to fragment size) are size standards to help with the assignment of the fragment sizes. CP, coat protein; SNP, single nucleotide polymorphism; WSMV, Wheat streak mosaic virus.