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Table 3 Primer details for Multiplex 3.

From: Multiplex genotyping system for efficient inference of matrilineal genetic ancestry with continental resolution

  PCR amplification Single-base extension
Site Primer sequences (5'-3') Concentration (μM) Amplicon size (bp) Primer sequence (5'-3') (5' aspecific tail in lowercase italics) Concentration (μM) Length (nt) Orientation Alleles (dye)
290-291 F CGCTTTCCACACAGACATCA 0.350 94/92 (gact) 6 gac CCACACAGACATCATAACAAAA 0.350 49 F a (green), d (red)*
  R GGGTTTGGCAGAGATGTGTT 0.350       
2092 F TTGCCCACAGAACCCTCTAA 0.300 141 act(gact) 3 ga CTCTAAATCCCCTTGTAAATTTAA 0.400 41 F C (yellow), T (red)
  R AATTGGTGGCTGCTTTTAGG 0.300       
3330 F CAGTCAGAGGTTCAATTCCTCTT 0.100 142 act(gact) 7 CGATTAGAATGGGTACAATGAG 0.150 53 R C (blue), T (green)
  R GGGCCTTTGCGTAGTTGTAT 0.100       
3826 F TGAAGTCACCCTAGCCATCA 0.200 168 ct(gact) 3 GGTCATGATGGCAGGAGTA 0.200 33 R C (blue), T (green)
  R AAGGGGGTTCGGTTGGT 0.200       
6285 F GAGCAGGAACAGGTTGAACA 0.300 237 actgactga CCTGCTAAGGGAGGGTAGA 0.450 28 R A (red), G (yellow)
  R GGAGAGATAGGAGAAGTAGGACTGC 0.300       
6374   same amplicon as 6285    ctgact TTGATGGCCCCTAAGAT 0.300 23 R C (blue), T (green)
11177 F TTCACAGCCACAGAACTAATCA 0.200 164 ct(gact) 10 gac GCGTTCAGGCGTTCTG 0.600 61 R C (blue), T (green)
  R AGTGCGATGAGTAGGGGAAG 0.200       
11365 F CACCCTAGGCTCACTAAACATTC 0.100 160 t(gact) 9 g CTTAATATGACTAGCTTACACAATAGC 0.100 65 F C (yellow), T (red)
  R TTCGACATGGGCTTTAGGG 0.100       
11959 F CCCCCACTATTAACCTACTGG 0.200 190 act(gact) 10 gac CTCCTACTTACAGGACTCAACAT 0.150 69 F A (green), G (blue)
  R TCTCGTGTGAATGAGGGTTTT 0.200       
12007   same amplicon as 11959    ct(gact) 9 gac GGTGGGTGAGTGAGCC 0.300 57 R A (red), G (yellow)
14433 F CTCCATCGCTAACCCCACTA 0.400 170 (gact) 6 GCTATTGAGGAGTATCCTGAG 0.300 45 R C (blue), T (green)
  R TTCTGAATTTTGGGGGAGGT 0.400       
14502   same amplicon as 14433    act(gact) 3 ga CATCATTCCCCCTAAATAAA 0.450 37 F C (yellow), T (red)
  1. * a, ancestral; d, deletion.
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Investigative Genetics

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