Table 2 Primer details for Multiplex 2.
| Â | PCR amplification | Single-base extension | |||||||
|---|---|---|---|---|---|---|---|---|---|
Site | Primer sequences (5'-3') | Concentration (μM) | Amplicon size (bp) | Primer sequence (5'-3') (5' aspecific tail in lowercase italics) | Concentration (μM) | Length (nt) | Orientation | Alleles (dye) | |
2706 | F | CGAGGGTTCAGCTGTCTCTT | 0.040 | 88 | (gact) 10 GTCTTCTCGTCTTGCTGTGT | 0.100 | 60 | R | A (red), G (yellow) |
| Â | R | AGGGTCTTCTCGTCTTGCTG | 0.040 | Â | Â | Â | Â | Â | Â |
3348 | F | CAGTCAGAGGTTCAATTCCTCTT | 0.200 | 142 | act(gact) 11 g GGAATGCCATTGCGAT | 0.200 | 64 | R | A (red), G (yellow) |
| Â | R | GGGCCTTTGCGTAGTTGTAT | 0.200 | Â | Â | Â | Â | Â | Â |
3480 | F | CGCTGACGCCATAAAACTCT | 0.120 | 120 | act(gact) 2 ga GCCATAAAACTCTTCACCAA | 0.150 | 33 | F | A (green), G (blue) |
| Â | R | AGGGGGGTTCATAGTAGAAG | 0.120 | Â | Â | Â | Â | Â | Â |
8281-8289 | F | GAAATCTGTGGAGCAAACCAC | 0.500 | 179/170 | (gact) 2 g CCCTATAGCACCCCCTCTA | 0.700 | 28 | F | a (yellow), d (blue)* |
| Â | R | AGAGGTGTTGGTTCTCTTAATCTTT | 0.500 | Â | Â | Â | Â | Â | Â |
11251 | F | TGAACGCAGGCACATACTTC | 0.100 | 92 | t(gact) 5 gac CCCCTACTCATCGCACT | 0.050 | 41 | F | A (green), G (blue) |
| Â | R | TGAGCCTAGGGTGTTGTGAG | 0.100 | Â | Â | Â | Â | Â | Â |
11719 | F | GGCGCAGTCATTCTCATAATC | 0.100 | 85 | (gact) 6 GCAGAATAGTAATGAGGATGTAAG | 0.150 | 48 | R | A (red), G (yellow) |
| Â | R | TGTGAGTGCGTTCGTAGTTTG | 0.100 | Â | Â | Â | Â | Â | Â |
12308 | F | CAGCTATCCATTGGTCTTAGGC | 2.000 | 169 | t(gact) 9 gac TGGTCTTAGGCCCCAA | 3.000 | 56 | F | A (green), G (blue) |
| Â | R | GATTTTACATAATGGGGGTATGAGT | 2.000 | Â | Â | Â | Â | Â | Â |
12705 | F | ACTTCTCCATAATATTCATCCCTGT | 1.300 | 184 | act(gact) 5 g TTAATCAGTTCTTCAAATATCTACTCAT | 0.800 | 52 | F | C (yellow), T (red) |
| Â | R | TCTCAGCCGATGAACAGTTG | 1.300 | Â | Â | Â | Â | Â | Â |
13368 | F | CGCCTTCTTCAAAGCCATAC | 0.250 | 127 | ct(gact) 2 gac TAAGGTTGTGGATGATGGA | 0.300 | 32 | R | A (red), G (yellow) |
| Â | R | GGTGAGGGAGGTTGAAGTGA | 0.250 | Â | Â | Â | Â | Â | Â |
13928 | F | CAGCCCTAGACCTCAACTACCT | 0.040 | 119 | ct(gact) 5 ga AACATACTCGGATTCTACCCTA | 0.040 | 46 | F | C (yellow), G (blue) |
| Â | R | ATAGGGGATTGTGCGGTGT | 0.040 | Â | Â | Â | Â | Â | Â |
14766 | F | TCAACTACAAGAACACCAATGACC | 0.050 | 109 | c GACCCCAATACGCAAAA | 0.150 | 18 | F | C (yellow), T (red) |
| Â | R | ATCATGCGGAGATGTTGGAT | 0.050 | Â | Â | Â | Â | Â | Â |
15904 | F | CATCCGTACTATACTTCACAACAATC | 1.000 | 184 | act(gact) 4 GGCCTGTCCTTGTAGTATAAA | 0.600 | 40 | F | C (yellow), T (red) |
| Â | R | GGTGCTAATGGTGGAGTTAAAGA | 1.000 | Â | Â | Â | Â | Â | Â |