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Table 2 Primer details for Multiplex 2.

From: Multiplex genotyping system for efficient inference of matrilineal genetic ancestry with continental resolution

  PCR amplification Single-base extension
Site Primer sequences (5'-3') Concentration (μM) Amplicon size (bp) Primer sequence (5'-3') (5' aspecific tail in lowercase italics) Concentration (μM) Length (nt) Orientation Alleles (dye)
2706 F CGAGGGTTCAGCTGTCTCTT 0.040 88 (gact) 10 GTCTTCTCGTCTTGCTGTGT 0.100 60 R A (red), G (yellow)
  R AGGGTCTTCTCGTCTTGCTG 0.040       
3348 F CAGTCAGAGGTTCAATTCCTCTT 0.200 142 act(gact) 11 g GGAATGCCATTGCGAT 0.200 64 R A (red), G (yellow)
  R GGGCCTTTGCGTAGTTGTAT 0.200       
3480 F CGCTGACGCCATAAAACTCT 0.120 120 act(gact) 2 ga GCCATAAAACTCTTCACCAA 0.150 33 F A (green), G (blue)
  R AGGGGGGTTCATAGTAGAAG 0.120       
8281-8289 F GAAATCTGTGGAGCAAACCAC 0.500 179/170 (gact) 2 g CCCTATAGCACCCCCTCTA 0.700 28 F a (yellow), d (blue)*
  R AGAGGTGTTGGTTCTCTTAATCTTT 0.500       
11251 F TGAACGCAGGCACATACTTC 0.100 92 t(gact) 5 gac CCCCTACTCATCGCACT 0.050 41 F A (green), G (blue)
  R TGAGCCTAGGGTGTTGTGAG 0.100       
11719 F GGCGCAGTCATTCTCATAATC 0.100 85 (gact) 6 GCAGAATAGTAATGAGGATGTAAG 0.150 48 R A (red), G (yellow)
  R TGTGAGTGCGTTCGTAGTTTG 0.100       
12308 F CAGCTATCCATTGGTCTTAGGC 2.000 169 t(gact) 9 gac TGGTCTTAGGCCCCAA 3.000 56 F A (green), G (blue)
  R GATTTTACATAATGGGGGTATGAGT 2.000       
12705 F ACTTCTCCATAATATTCATCCCTGT 1.300 184 act(gact) 5 g TTAATCAGTTCTTCAAATATCTACTCAT 0.800 52 F C (yellow), T (red)
  R TCTCAGCCGATGAACAGTTG 1.300       
13368 F CGCCTTCTTCAAAGCCATAC 0.250 127 ct(gact) 2 gac TAAGGTTGTGGATGATGGA 0.300 32 R A (red), G (yellow)
  R GGTGAGGGAGGTTGAAGTGA 0.250       
13928 F CAGCCCTAGACCTCAACTACCT 0.040 119 ct(gact) 5 ga AACATACTCGGATTCTACCCTA 0.040 46 F C (yellow), G (blue)
  R ATAGGGGATTGTGCGGTGT 0.040       
14766 F TCAACTACAAGAACACCAATGACC 0.050 109 c GACCCCAATACGCAAAA 0.150 18 F C (yellow), T (red)
  R ATCATGCGGAGATGTTGGAT 0.050       
15904 F CATCCGTACTATACTTCACAACAATC 1.000 184 act(gact) 4 GGCCTGTCCTTGTAGTATAAA 0.600 40 F C (yellow), T (red)
  R GGTGCTAATGGTGGAGTTAAAGA 1.000       
  1. * a, ancestral; d, deletion.
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Investigative Genetics

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