Table 1 Primer details for Multiplex 1.
| Â | PCR amplification | Single-base extension | |||||||
|---|---|---|---|---|---|---|---|---|---|
Site | Primer sequences (5'-3') | Concentration (μM) | Amplicon size (bp) | Primer sequence (5'-3') (5' aspecific tail in lowercase italics) | Concentration (μM) | Length (nt) | Orientation | Alleles (dye) | |
769 | F | ACATCACCCCATAAACAAATAGG | 1.000 | 158 | act(gact) 10 CGTTTTGAGCTGCATTG | 2.000 | 60 | R | A (red), G (yellow) |
| Â | R | AGCGTTTTGAGCTGCATTG | 1.000 | Â | Â | Â | Â | Â | Â |
1243 | F | AATCGATAAACCCCGATCAA | 0.040 | 93 | actgact CGATCAACCTCACCACC | 0.150 | 24 | F | C (yellow), T (red) |
| Â | R | TGCGCTTACTTTGTAGCCTTC | 0.040 | Â | Â | Â | Â | Â | Â |
1736 | F | GCTAAACCTAGCCCCAAACC | 0.200 | 106 | t(gact) 2 gac TCAATTTCTATCGCCTATACTTTAT | 0.150 | 37 | R | A (red), G (yellow) |
| Â | R | CTATTGCGCCAGGTTTCAAT | 0.200 | Â | Â | Â | Â | Â | Â |
3552 | F | CGCTGACGCCATAAAACTCT | 4.000 | 128 | actgact AGGGGGGTTCATAGTAGAAG | 4.000 | 27 | R | A (red), C (blue), T (green) |
| Â | R | GTATGGGGAGGGGGGTTC | 4.000 | Â | Â | Â | Â | Â | Â |
4883 | F | TGACATCCGGCCTGCTT | 0.075 | 114 | (gact) 4 g CATGACAAAAACTAGCCCC | 0.300 | 36 | F | C (yellow), T (red) |
| Â | R | TGGATAAGATTGAGAGAGTGAGGA | 0.075 | Â | Â | Â | Â | Â | Â |
7146 | F | AGACCAAACCTACGCCAAAA | 0.150 | 130 | ct(gact) 9 ga TACGCCAAAATCCATTTC | 0.300 | 58 | F | A (green), G (blue) |
| Â | R | GGTGTATGCATCGGGGTAGT | 0.150 | Â | Â | Â | Â | Â | Â |
10034 | F | TCTCCATCTATTGATGAGGGTCT | 0.300 | 108 | ct(gact) 4 g GTACCGTTAACTTCCAATTAACTAG | 0.150 | 44 | F | C (yellow), T (red) |
| Â | R | TTAAGGCGAAGTTTATTACTCTTTTT | 0.300 | Â | Â | Â | Â | Â | Â |
10238 | F | GCGTCCCTTTCTCCATAAAA | 0.250 | 80 | ct(gact) 4 gac CTCCATAAAATTCTTCTTAGTAGCTAT | 0.300 | 48 | F | C (yellow), T (red) |
| Â | R | GGGTAAAAGGAGGGCAATTT | 0.250 | Â | Â | Â | Â | Â | Â |
10400 | F | GCCCTAAGTCTGGCCTATGA | 0.150 | 90 | ct(gact) 5 gac CGTTTTGTTTAAACTATATACCAATTC | 0.300 | 52 | R | C (blue), T (green) |
| Â | R | TGAGTCGAAATCATTCGTTTT | 0.150 | Â | Â | Â | Â | Â | Â |
12705 | F | CCCAAACATTAATCAGTTCTTCAA | 0.180 | 102 | t(gact) 6 g TTAATCAGTTCTTCAAATATCTACTCAT | 0.150 | 54 | F | C (yellow), T (red) |
| Â | R | TCTCAGCCGATGAACAGTTG | 0.180 | Â | Â | Â | Â | Â | Â |
12950 | F | TCCTCGCCTTAGCATGATTT | 0.200 | 101 | act(gact) 11 TGAGGCTTGGATTAGCG | 0.500 | 64 | R | A (red), C (blue), G (yellow) |
| Â | R | GAGGCCTAGTAGTGGGGTGA | 0.200 | Â | Â | Â | Â | Â | Â |
13966 | F | ACCGCACAATCCCCTATCTA | 0.250 | 132 | ct(gact) 13 GCAGGTTTTGGCTCG | 0.500 | 69 | R | A (red), G (yellow) |
| Â | R | AGGTGATGATGGAGGTGGAG | 0.250 | Â | Â | Â | Â | Â | Â |
15301 | F | CCACCCTCACACGATTCTTT | 0.080 | 119 | ct(gact) 4 ATTCTTTACCTTTCACTTCATCTT | 0.150 | 42 | F | A (green), G (blue) |
| Â | R | GGTGATTCCTAGGGGGTTGT | 0.080 | Â | Â | Â | Â | Â | Â |