Feather barbs as a good source of mtDNA for bird species identification in forensic wildlife investigations

Investigative Genetics

Table 2 Results of PCR amplifications, sequencing and species identifications for two-barb and five-barb feather samples

Code	Sample	Barbs, n	Extraction method	Sequence quality	Species (Genbank accession number)
FE1	Wild turkey (fresh)	5	MSSC	Clear	Meleagris gallopavo (JF303667)
FE2	Wild turkey (fresh)	5	MSSC	Clear
FE3	Wild turkey (fresh)	5	QDIK	Clear
FE4	Wild turkey (fresh)	5	QDIK	Clear
FE5	Canada goose (fresh)	5	MSSC	Clear	Branta canadensis (JF303668)
FE6	Canada goose (fresh)	5	MSSC	Clear
FE7	Canada goose (fresh)-	5	QDIK	Clear
FE8	Canada goose (fresh)	5	QDIK	Clear
FE9	Wild turkey (fresh)	2	MSSC	Clear	M. gallopavo (JF303667)
FE10	Wild turkey (fresh)	2	MSSC	Clear
FE11	Wild turkey (fresh)	2	QDIK	Clear
FE12	Wild turkey (fresh)	2	QDIK	Clear
FE13	Canada goose (fresh)	2	MSSC	Clear	B. canadensis (JF303668)
FE14	Canada goose (fresh)	2	MSSC	Clear
FE15	Canada goose (fresh)	2	QDIK	Clear
FE16	Canada goose (fresh)	2	QDIK	Clear
FE17	Ruffled grouse (museum)	5	MSSC	Clear	Bonasa umbellus (JF303669)
FE18	Ruffled grouse (museum)	2	MSSC	Mixed peaks
FE19	Blue heron (stored)	5	MSSC	Clear	Ardea herodias (JF303671)
FE20	Blue heron (stored)	2	MSSC	Clear
FE21	Pygmy owl (stored)	5	MSSC	Clear	Glaucidium californicum (JF303670)
FE22	Pygmy owl (stored)	2	MSSC	Clear
EG1	Magpie (archaeological)	2	MSSC	Clear	Pica pica (JF303672)
EG2	Magpie (archaeological)	5	MSSC	Clear

ISSN: 2041-2223