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Table 3 Data for swabs containing mixtures of epithelial cells and semen from volunteer 2 (data are mean ± SD unless otherwise stated).

From: Differential DNA extraction of challenging simulated sexual-assault samples: a Swiss collaborative study

Laboratory

Male DNA in SF, nga

Female DNA in SF, nga

Male:female ratio

Extract volume of SF, μl

Male DN in EF, nga

Female DNA in EF, nga

Recovered male DNA in SF, %b

SGM Plusc profiles (SF), ngd

Y loci, nf

1

2.2 ± 1.2

5.6 ± 6.1

1:3

25

0.8 ± 0.1

2584.0 ± 790.1

10

M + F (major)

11 (SF)

2

0.1 ± 0.1

1.5 ± 0.3

1:12

40

3.6 ± 1.7

3408.9 ± 1457.6

1

Not interpretable

11 (EF)

3

3.5 ± 0.2

1.7 ± 0.3

2:1

25

2.0 ± 0.3

2875.7 ± 602.6

16

M (major) + F

11 (SF)

4

0.8 ± 0.7

0.6 ± 2.4

1:1

22

1.6 ± 0.4

3836.6 ± 1454.3

4

Not interpretable

11 (EF)

5

NDe

1.2 ± 0.7

0

50

ND

0.1 ± 0.0

0

Not interpretable

0 (SF/EF)

6

0.2 ± 0.0

0.8 ± 1.1

1:3

60

0.2 ± 0.2

500.2 ± 333.7

1

Not interpretable

7 (SF/EF)

7

NDe

0.4 ± 0.0

0

25

ND

67.3 ± 17.3

0

Not interpretable

7 (SF/EF)

8

1.7 ± 0.1

1.9 ± 0.5

1:1

25

ND

248.3 ± 22.9

8

Not interpretable

11 (SF)

9

0.3 ± 0.4

0.2 ± 0.9

2:1

50

1.3 ± 0.4

5713.0 ± 394.6

1

Not interpretable

8 (EF)

  1. Relevant median values are in bold.
  2. aMean quantities and percentages of male and female DNA recovered in the sperm (SF) and epithelial-cell fractions (EF) after differential DNA extraction are reported (means are from duplicate analyses of two different samples, that is, four values).
  3. b100% correspond to the amount of male DNA recovered after direct DNA extraction of samples containing only semen, without epithelial cells
  4. cSecond Generation Multiplex Plus® (Applied Biosystems).
  5. dCharacteristics of the SGM Plus DNA profiles (M = male DNA profile; M+F = mixed DNA profile; major = major component of the mixed DNA profile) and number of loci obtained with the PowerPlex Y kit are given.
  6. eND = none detected
  7. fPowerPlex Y System (Promega)