Skip to main content

Table 3 Data for swabs containing mixtures of epithelial cells and semen from volunteer 2 (data are mean ± SD unless otherwise stated).

From: Differential DNA extraction of challenging simulated sexual-assault samples: a Swiss collaborative study

Laboratory Male DNA in SF, nga Female DNA in SF, nga Male:female ratio Extract volume of SF, μl Male DN in EF, nga Female DNA in EF, nga Recovered male DNA in SF, %b SGM Plusc profiles (SF), ngd Y loci, nf
1 2.2 ± 1.2 5.6 ± 6.1 1:3 25 0.8 ± 0.1 2584.0 ± 790.1 10 M + F (major) 11 (SF)
2 0.1 ± 0.1 1.5 ± 0.3 1:12 40 3.6 ± 1.7 3408.9 ± 1457.6 1 Not interpretable 11 (EF)
3 3.5 ± 0.2 1.7 ± 0.3 2:1 25 2.0 ± 0.3 2875.7 ± 602.6 16 M (major) + F 11 (SF)
4 0.8 ± 0.7 0.6 ± 2.4 1:1 22 1.6 ± 0.4 3836.6 ± 1454.3 4 Not interpretable 11 (EF)
5 NDe 1.2 ± 0.7 0 50 ND 0.1 ± 0.0 0 Not interpretable 0 (SF/EF)
6 0.2 ± 0.0 0.8 ± 1.1 1:3 60 0.2 ± 0.2 500.2 ± 333.7 1 Not interpretable 7 (SF/EF)
7 NDe 0.4 ± 0.0 0 25 ND 67.3 ± 17.3 0 Not interpretable 7 (SF/EF)
8 1.7 ± 0.1 1.9 ± 0.5 1:1 25 ND 248.3 ± 22.9 8 Not interpretable 11 (SF)
9 0.3 ± 0.4 0.2 ± 0.9 2:1 50 1.3 ± 0.4 5713.0 ± 394.6 1 Not interpretable 8 (EF)
  1. Relevant median values are in bold.
  2. aMean quantities and percentages of male and female DNA recovered in the sperm (SF) and epithelial-cell fractions (EF) after differential DNA extraction are reported (means are from duplicate analyses of two different samples, that is, four values).
  3. b100% correspond to the amount of male DNA recovered after direct DNA extraction of samples containing only semen, without epithelial cells
  4. cSecond Generation Multiplex Plus® (Applied Biosystems).
  5. dCharacteristics of the SGM Plus DNA profiles (M = male DNA profile; M+F = mixed DNA profile; major = major component of the mixed DNA profile) and number of loci obtained with the PowerPlex Y kit are given.
  6. eND = none detected
  7. fPowerPlex Y System (Promega)