|
Laboratory
|
Male DNA in SF, nga
|
Female DNA in SF, nga
|
Male:female ratio
|
Extract volume of SF, μl
|
Male DN in EF, nga
|
Female DNA in EF, nga
|
Recovered male DNA in SF, %b
|
SGM Plusc profiles (SF), ngd
|
Y loci, nf
|
|---|
|
1
|
2.2 ± 1.2
|
5.6 ± 6.1
|
1:3
|
25
|
0.8 ± 0.1
|
2584.0 ± 790.1
|
10
|
M + F (major)
|
11 (SF)
|
|
2
|
0.1 ± 0.1
|
1.5 ± 0.3
|
1:12
|
40
|
3.6 ± 1.7
|
3408.9 ± 1457.6
|
1
|
Not interpretable
|
11 (EF)
|
|
3
|
3.5 ± 0.2
|
1.7 ± 0.3
|
2:1
|
25
|
2.0 ± 0.3
|
2875.7 ± 602.6
|
16
|
M (major) + F
|
11 (SF)
|
|
4
|
0.8 ± 0.7
|
0.6 ± 2.4
|
1:1
|
22
|
1.6 ± 0.4
|
3836.6 ± 1454.3
|
4
|
Not interpretable
|
11 (EF)
|
|
5
|
NDe
|
1.2 ± 0.7
|
0
|
50
|
ND
|
0.1 ± 0.0
|
0
|
Not interpretable
|
0 (SF/EF)
|
|
6
|
0.2 ± 0.0
|
0.8 ± 1.1
|
1:3
|
60
|
0.2 ± 0.2
|
500.2 ± 333.7
|
1
|
Not interpretable
|
7 (SF/EF)
|
|
7
|
NDe
|
0.4 ± 0.0
|
0
|
25
|
ND
|
67.3 ± 17.3
|
0
|
Not interpretable
|
7 (SF/EF)
|
|
8
|
1.7 ± 0.1
|
1.9 ± 0.5
|
1:1
|
25
|
ND
|
248.3 ± 22.9
|
8
|
Not interpretable
|
11 (SF)
|
|
9
|
0.3 ± 0.4
|
0.2 ± 0.9
|
2:1
|
50
|
1.3 ± 0.4
|
5713.0 ± 394.6
|
1
|
Not interpretable
|
8 (EF)
|
- Relevant median values are in bold.
- aMean quantities and percentages of male and female DNA recovered in the sperm (SF) and epithelial-cell fractions (EF) after differential DNA extraction are reported (means are from duplicate analyses of two different samples, that is, four values).
- b100% correspond to the amount of male DNA recovered after direct DNA extraction of samples containing only semen, without epithelial cells
- cSecond Generation Multiplex Plus® (Applied Biosystems).
- dCharacteristics of the SGM Plus DNA profiles (M = male DNA profile; M+F = mixed DNA profile; major = major component of the mixed DNA profile) and number of loci obtained with the PowerPlex Y kit are given.
- eND = none detected
- fPowerPlex Y System (Promega)
