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Table 2 Data for swabs containing mixtures of epithelial cells and semen from volunteer 1 (data are mean ± SD unless otherwise stated).

From: Differential DNA extraction of challenging simulated sexual-assault samples: a Swiss collaborative study

Laboratory Male DNA in SF, nga Female DNA in SF, nga Male:female ratio Extract volume of SF, μl Male DNA in EF, nga Female DNA in EF, nga Recovered male DNA in SF, %b SGM Plusc profiles (SF), ngd Y loci, ng
1 26.5 ± 7.3 6.0 ± 17.3 5:1 25 3.3 ± 1.1 3615.1 ± 78.6 14 M (major) + F 11 (SF)
2 3.3 ± 2.2 4.7 ± 7.4 1:1 40 7.4 ± 0.5 6729.6 ± 2628.1 2 M (major) + F 11 (EF)
3 18.1 ± 0.9 8.4 ± 16.3 2:1 25 4.7 ± 0.5 5367.0 ± 2352.6 10 M (major) + F 11 (SF)
4 13.0 ± 10.9 5.8 ± 27.6 2:1 22 4.3 ± 0.1 3801.7 ± 545.7 7 M 11 (SF)
5 NDe 1.5 ± 1.1 0 50 0.4 ± 0.1 0.1 ± 0.2 0 Not interpretable 8 (EF)
6 0.4 ± 0.2 2.1 ± 0.6 1:6 60 0.2 ± 0.2 45.1 ± 28.9 0 Not interpretable 11 (SF)
7 0.8 ± 0.2 ND Indet.f 25 0.1 ± 0.2 50.0 ± 13.4 0 Not interpretable 11 (SF)
8 11.4 ± 5.8 9.7 ± 13.7 1:1 25 ND 387.8 ± 494.9 6 M (major) + F 11 (SF)
9 10.3 ± 2.0 1.2 ± 4.0 9:1 50 3.4 ± 1.8 5765.2 ± 192.1 6 M 11 (SF)
  1. Relevant median values are in bold.
  2. aMean quantities and percentages of male and female DNA recovered in the sperm (SF) and epithelial-cell fractions (EF) after differential DNA extraction are reported (means are from duplicate analyses of two different samples, that is, four values).
  3. b100% correspond to the amount of male DNA recovered after direct DNA extraction of samples containing only semen, without epithelial cells
  4. cSecond Generation Multiplex Plus® (Applied Biosystems).
  5. dCharacteristics of the SGM Plus DNA profiles (M = male DNA profile; M+F = mixed DNA profile; major = major component of the mixed DNA profile) and number of loci obtained with the PowerPlex Y kit are given.
  6. eND = none detected
  7. fIndet. = indeterminate.
  8. gPowerPlex Y System (Promega)