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Table 2 Data for swabs containing mixtures of epithelial cells and semen from volunteer 1 (data are mean ± SD unless otherwise stated).

From: Differential DNA extraction of challenging simulated sexual-assault samples: a Swiss collaborative study

Laboratory

Male DNA in SF, nga

Female DNA in SF, nga

Male:female ratio

Extract volume of SF, μl

Male DNA in EF, nga

Female DNA in EF, nga

Recovered male DNA in SF, %b

SGM Plusc profiles (SF), ngd

Y loci, ng

1

26.5 ± 7.3

6.0 ± 17.3

5:1

25

3.3 ± 1.1

3615.1 ± 78.6

14

M (major) + F

11 (SF)

2

3.3 ± 2.2

4.7 ± 7.4

1:1

40

7.4 ± 0.5

6729.6 ± 2628.1

2

M (major) + F

11 (EF)

3

18.1 ± 0.9

8.4 ± 16.3

2:1

25

4.7 ± 0.5

5367.0 ± 2352.6

10

M (major) + F

11 (SF)

4

13.0 ± 10.9

5.8 ± 27.6

2:1

22

4.3 ± 0.1

3801.7 ± 545.7

7

M

11 (SF)

5

NDe

1.5 ± 1.1

0

50

0.4 ± 0.1

0.1 ± 0.2

0

Not interpretable

8 (EF)

6

0.4 ± 0.2

2.1 ± 0.6

1:6

60

0.2 ± 0.2

45.1 ± 28.9

0

Not interpretable

11 (SF)

7

0.8 ± 0.2

ND

Indet.f

25

0.1 ± 0.2

50.0 ± 13.4

0

Not interpretable

11 (SF)

8

11.4 ± 5.8

9.7 ± 13.7

1:1

25

ND

387.8 ± 494.9

6

M (major) + F

11 (SF)

9

10.3 ± 2.0

1.2 ± 4.0

9:1

50

3.4 ± 1.8

5765.2 ± 192.1

6

M

11 (SF)

  1. Relevant median values are in bold.
  2. aMean quantities and percentages of male and female DNA recovered in the sperm (SF) and epithelial-cell fractions (EF) after differential DNA extraction are reported (means are from duplicate analyses of two different samples, that is, four values).
  3. b100% correspond to the amount of male DNA recovered after direct DNA extraction of samples containing only semen, without epithelial cells
  4. cSecond Generation Multiplex Plus® (Applied Biosystems).
  5. dCharacteristics of the SGM Plus DNA profiles (M = male DNA profile; M+F = mixed DNA profile; major = major component of the mixed DNA profile) and number of loci obtained with the PowerPlex Y kit are given.
  6. eND = none detected
  7. fIndet. = indeterminate.
  8. gPowerPlex Y System (Promega)