|
Laboratory
|
Male DNA in SF, nga
|
Female DNA in SF, nga
|
Male:female ratio
|
Extract volume of SF, μl
|
Male DNA in EF, nga
|
Female DNA in EF, nga
|
Recovered male DNA in SF, %b
|
SGM Plusc profiles (SF), ngd
|
Y loci, ng
|
|---|
|
1
|
26.5 ± 7.3
|
6.0 ± 17.3
|
5:1
|
25
|
3.3 ± 1.1
|
3615.1 ± 78.6
|
14
|
M (major) + F
|
11 (SF)
|
|
2
|
3.3 ± 2.2
|
4.7 ± 7.4
|
1:1
|
40
|
7.4 ± 0.5
|
6729.6 ± 2628.1
|
2
|
M (major) + F
|
11 (EF)
|
|
3
|
18.1 ± 0.9
|
8.4 ± 16.3
|
2:1
|
25
|
4.7 ± 0.5
|
5367.0 ± 2352.6
|
10
|
M (major) + F
|
11 (SF)
|
|
4
|
13.0 ± 10.9
|
5.8 ± 27.6
|
2:1
|
22
|
4.3 ± 0.1
|
3801.7 ± 545.7
|
7
|
M
|
11 (SF)
|
|
5
|
NDe
|
1.5 ± 1.1
|
0
|
50
|
0.4 ± 0.1
|
0.1 ± 0.2
|
0
|
Not interpretable
|
8 (EF)
|
|
6
|
0.4 ± 0.2
|
2.1 ± 0.6
|
1:6
|
60
|
0.2 ± 0.2
|
45.1 ± 28.9
|
0
|
Not interpretable
|
11 (SF)
|
|
7
|
0.8 ± 0.2
|
ND
|
Indet.f
|
25
|
0.1 ± 0.2
|
50.0 ± 13.4
|
0
|
Not interpretable
|
11 (SF)
|
|
8
|
11.4 ± 5.8
|
9.7 ± 13.7
|
1:1
|
25
|
ND
|
387.8 ± 494.9
|
6
|
M (major) + F
|
11 (SF)
|
|
9
|
10.3 ± 2.0
|
1.2 ± 4.0
|
9:1
|
50
|
3.4 ± 1.8
|
5765.2 ± 192.1
|
6
|
M
|
11 (SF)
|
- Relevant median values are in bold.
- aMean quantities and percentages of male and female DNA recovered in the sperm (SF) and epithelial-cell fractions (EF) after differential DNA extraction are reported (means are from duplicate analyses of two different samples, that is, four values).
- b100% correspond to the amount of male DNA recovered after direct DNA extraction of samples containing only semen, without epithelial cells
- cSecond Generation Multiplex Plus® (Applied Biosystems).
- dCharacteristics of the SGM Plus DNA profiles (M = male DNA profile; M+F = mixed DNA profile; major = major component of the mixed DNA profile) and number of loci obtained with the PowerPlex Y kit are given.
- eND = none detected
- fIndet. = indeterminate.
- gPowerPlex Y System (Promega)
