Validation of amplification in Y. pestis strain La Paz. The amplified region is depicted as in Figure 1. Positions of IS 1661 elements on the CO92 chromosome are indicated by cyan bars flanking the amplification. Inset: TaqMan® assays were designed, two internal to the amplified region and one to each side, with the approximate coordinates indicated on the X-axis. These assays were run in triplicate on genomic DNA from each of the two strains, and relative fold-change of La Paz versus CO92 was calculated and normalized to the assay at coordinate 4,560,000.