Forensic identification of severely degraded Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) tissues

Table 3 Overview of PCR primer sets used in this study

Primer name	Sequence 5" to 3"	T_a, °C^a	Amplicon size, bp
Short fragment COI primers
OmyCOI032F	ATAGTAGGCACCGCCCTGAGTCTAC	62.0	52
OmyCOI032R	CCGGCTGGCTTAGTTCCGCC
OmyCOI203F	CTAATAATCGGAGCCCCTGATATG	62.0	73
OmyCOI203R	AGGATGGAGGAAGGAGTCAGAAG
OmyCOI340F	CCCTCTAGCCGGCAACCTC	62.0	62
OmyCOI340R	GAAGGGAGAAGATAGTTAAATCAACAGAG
SsaCOI030F	GAATAGTCGGCACCGCCCTAAGTCTCT	62.0	57
SsaCOI030R	CGCCAGGCTGGCTGAGTTCTGCT
SsaCOI208F	AATCGGGGCCCCCGACATA	62.0	71
SsaCOI208R	GAAAGGAGGGAGGGAGAAGTCAAAAA
SsaCOI334F	CTACCCCCCTCTAGCAGGTAATCTT	62.0	65
SsaCOI334R	GGGAAAAAATAGTTAAGTCAACGGAA
SsaCOI076F	CCAGCCTGGCGCCCTTCTG	62.0	63
SsaCOI076R	AAGGCATGGGCTGTAACAATTACGTT
SsaCOI213F	GGGCCCCCGACATAGCAT	62.0	65
SsaCOI213R	AAAGGAGGGAGGAGAAGTCAAAAA
SsaCOI401F	CATTTGGCTGGTATTTCTTCAATTCTT	62.0	75
SsaCOI401R	AGCTGGGGGTTTTATATTAATAATGGTT
Barcoding COI primers ^b
Fish1F	TCAACCAACCACAAAGACATTGGCAC	55.0	704
Fish1R	TAGACTTCTGGGTGGCCAAAGAATCA
Microsatellite primers
Omm1303F ^c	GGAAGGAAAGGCACTT	55.0	285 to 375^e
Omm1303R ^c	TCTACACCAGGAGAGAGTAAT
Sp2201F ^d	TTAGATGGTGGGATACTGGGAGGC	55.0	250 to 350^e
Sp2201R ^d	CGGGAGCCCCATAACCCTACTAATAAC

^aT_a = the routine PCR annealing temperature used for each primer set;
^buniversal barcoding primers [37]; ^crainbow trout microsatellite [39]; ^dAtlantic salmon microsatellite [38]; ^emicrosatellite allele sizes were derived from farmed fish surveys.

ISSN: 2041-2223